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Title: Chromosome doubling method

Patent ·
OSTI ID:1175991

The invention provides methods for chromosome doubling in plants. The technique overcomes the low yields of doubled progeny associated with the use of prior techniques for doubling chromosomes in plants such as grasses. The technique can be used in large scale applications and has been demonstrated to be highly effective in maize. Following treatment in accordance with the invention, plants remain amenable to self fertilization, thereby allowing the efficient isolation of doubled progeny plants.

Research Organization:
Univ. of Missouri, Columbia, MO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
C-5-32680
Assignee:
University Of Missouri
Patent Number(s):
7,135,615
Application Number:
10/164,362
OSTI ID:
1175991
Country of Publication:
United States
Language:
English

References (39)

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Genetic Control of Cell Division Patterns in Developing Plants journal February 1997
Morphology, Cytology, and Fertility of F 1 and Induced‐amphiploid Hybrids of Elymus canadensisAgropyron subsecundum 1 journal January 1977
Hematoxylin Procedure for Staining Mature Pollen Grains in Maize with Dimethylsulfoxide as a Clearing Agent journal January 1998
Nitrous oxide as a possible alternative agent for chromosome doubling of wheat haploids journal January 1988
Characterization of Autotetraploid Russian Wildrye Produced with Nitrous Oxide journal September 1991
Anther Culture of Elite Genotypes of Maize 1 journal September 1986
Increased doubled haploid plant regeneration from rice (Oryza sativa L.) anthers cultured on colchicine-supplemented media journal May 1994
Antimicrotubule herbicides for in vitro chromosome doubling in Beta vulgaris L. ovule culture journal June 1998
Direct effect of colchicine on the microspore embryogenesis to produce dihaploid plants in wheat (Triticum aestivum L.) journal May 1991
In vivo haploid induction in maize journal March 2002
Chromosome doubling of haploid maize seedlings using nitrous oxide gas at the flower primordial stage journal October 2002
PRODUCTION OF ANEUPLOIDS IN AVENA SATIVA L. BY NITROUS OXIDE journal September 1973

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