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Title: Protein crystallography prescreen kit

Abstract

A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

Inventors:
; ;
Publication Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175418
Patent Number(s):
6,916,455
Assignee:
University Of California, The Regents Of
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Segelke, Brent W., Krupka, Heike I., and Rupp, Bernhard. Protein crystallography prescreen kit. United States: N. p., 2005. Web.
Segelke, Brent W., Krupka, Heike I., & Rupp, Bernhard. Protein crystallography prescreen kit. United States.
Segelke, Brent W., Krupka, Heike I., and Rupp, Bernhard. 2005. "Protein crystallography prescreen kit". United States. https://www.osti.gov/servlets/purl/1175418.
@article{osti_1175418,
title = {Protein crystallography prescreen kit},
author = {Segelke, Brent W. and Krupka, Heike I. and Rupp, Bernhard},
abstractNote = {A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.},
doi = {},
url = {https://www.osti.gov/biblio/1175418}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 12 00:00:00 EDT 2005},
month = {Tue Jul 12 00:00:00 EDT 2005}
}