Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme

Chowdhury, Sayan Mullick; Surhland, Cassandra; Sanchez, Zina; Chaudhary, Pankaj; Suresh Kumar, M. A.; Lee, Stephen; Peña, Louis A.; Waring, Michael; Sitharaman, Balaji; Naidu, Mamta

doi:10.1016/j.nano.2014.08.001

Title: Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme

Journal Article · Wed Aug 13 00:00:00 EDT 2014 · Nanomedicine: Nanotechnologoy, Biology, and Medicine

DOI:https://doi.org/10.1016/j.nano.2014.08.001· OSTI ID:1174110

Chowdhury, Sayan Mullick ^[1]; Surhland, Cassandra ^[1]; Sanchez, Zina ^[2]; Chaudhary, Pankaj ^[3]; Suresh Kumar, M. A. ^[2]; Lee, Stephen ^[1]; Peña, Louis A. ^[4]; Waring, Michael ^[5]; Sitharaman, Balaji ^[1];

^[6]

Stony Brook Univ., NY (United States). Dept. of Biomedical Engineering
Stony Brook Univ., NY (United States). Dept. of Pharmacological Sciences
Queens Univ., Belfast, Ireland (United Kingdom). Centre for Cancer Research and Cell Biology
Stony Brook Univ., NY (United States). Dept. of Biomedical Engineering; Brookhaven National Lab. (BNL), Upton, NY (United States). Biosciences Dept.
Univ. of Cambridge (United Kingdom). Dept. of Pharmacology
Tufts School of Medicine, Boston, MA (United States). GeneSys Research Institute/Center for Cancer Systems Biology

We report use of PEG-DSPE coated oxidized graphene nanoribbons (O-GNR-PEG-DSPE) as agent for delivery of anti-tumor drug Lucanthone (Luc) into Glioblastoma Multiformae (GBM) cells targeting base excision repair enzyme APE-1 (Apurinic endonuclease-1). Lucanthone, an endonuclease inhibitor of APE-1, was loaded onto O-GNR-PEG-DSPEs using a simple non-covalent method. We found its uptake by GBM cell line U251 exceeding 67% and 60% in APE-1-overexpressing U251, post 24 hours (h). However, their uptake was ~38% and 29% by MCF-7 and rat glial progenitor cells (CG-4), respectively. TEM analysis of U251 showed large aggregates of O-GNR-PEG-DSPE in vesicles. Luc-O-GNR-PEG-DSPE was significantly toxic to U251 but showed little / no toxicity when exposed to MCF-7/CG-4 cells. This differential uptake effect can be exploited to use O-GNR-PEG-DSPEs as a vehicle for Luc delivery to GBM, while reducing nonspecific cytotoxicity to the surrounding healthy tissue. In conclusion, cell death in U251 was necrotic, probably due to oxidative degradation of APE-1.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC00112704; KP-1401020/MO-079; 1DP2OD007394-01

OSTI ID:: 1174110

Report Number(s):: BNL-107524-2015-JA; R&D Project: MO-079; KP1401020

Journal Information:: Nanomedicine: Nanotechnologoy, Biology, and Medicine, Vol. 11, Issue 1; ISSN 1549-9634

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 80 works

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Title: Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme

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