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Title: Overproduction of ligninolytic enzymes

Patent ·
OSTI ID:1134342

Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IBB 303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
ACO2-05CH11231
Assignee:
The Regents of The University of California (Oakland, CA)
Patent Number(s):
8,753,844
Application Number:
13/464,681
OSTI ID:
1134342
Resource Relation:
Patent File Date: 2012 May 04
Country of Publication:
United States
Language:
English

References (13)

Microbial method for producing lignin peroxidase patent October 1992
Process for the production of lignolytic enzymes by means of white rot fungi patent April 1995
Phanerochaete chrysospoirium strains CNCM I-1511, I-1512 and I-1513 for producing lignin peroxidase and manganese peroxidase patent October 1999
White rot-lignin-modifying fungus Flavodon flavus and a process for removing dye from dye containing water or soil using the fungus patent May 2002
Laccase enzymes and their uses patent June 2010
Degrading lignocellulosic materials patent-application May 2006
Interlaboratory testing of methods for assay of xylanase activity journal May 1992
Physiological regulation of laccase and manganese peroxidase production by white-rot Basidiomycetes journal October 2009
Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens journal April 2002
Measurement of cellulase activities journal January 1987
Effect of culture conditions on manganese peroxidase production and activity by some white rot fungi journal January 2003
Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties journal February 2010
Immobilization of laccase from Cerrena unicolor on controlled porosity glass journal January 1999