Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis
Abstract
Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.
- Inventors:
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1132269
- Patent Number(s):
- 8,728,290
- Application Number:
- 13/667,254
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Nov 02
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Sommer, Gregory J, Hatch, Anson V, Singh, Anup K, and Wang, Ying-Chih. Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis. United States: N. p., 2014.
Web.
Sommer, Gregory J, Hatch, Anson V, Singh, Anup K, & Wang, Ying-Chih. Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis. United States.
Sommer, Gregory J, Hatch, Anson V, Singh, Anup K, and Wang, Ying-Chih. 2014.
"Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis". United States. https://www.osti.gov/servlets/purl/1132269.
@article{osti_1132269,
title = {Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis},
author = {Sommer, Gregory J and Hatch, Anson V and Singh, Anup K and Wang, Ying-Chih},
abstractNote = {Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.},
doi = {},
url = {https://www.osti.gov/biblio/1132269},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 20 00:00:00 EDT 2014},
month = {Tue May 20 00:00:00 EDT 2014}
}
Works referenced in this record:
Polyacrylamide gel plugs enabling 2-D microfluidic protein separationsvia isoelectric focusing and multiplexed sodium dodecyl sulfate gel electrophoresis
journal, June 2008
- Liu, Jikun; Yang, Shuang; Lee, Cheng S.
- ELECTROPHORESIS, Vol. 29, Issue 11
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
- Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
- Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333