Interim report on updated microarray probes for the LLNL Burkholderia pseudomallei SNP array
The overall goal of this project is to forensically characterize 100 unknown Burkholderia isolates in the US-Australia collaboration. We will identify genome-wide single nucleotide polymorphisms (SNPs) from B. pseudomallei and near neighbor species including B. mallei, B. thailandensis and B. oklahomensis. We will design microarray probes to detect these SNP markers and analyze 100 Burkholderia genomic DNAs extracted from environmental, clinical and near neighbor isolates from Australian collaborators on the Burkholderia SNP microarray. We will analyze the microarray genotyping results to characterize the genetic diversity of these new isolates and triage the samples for whole genome sequencing. In this interim report, we described the SNP analysis and the microarray probe design for the Burkholderia SNP microarray.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 1047245
- Report Number(s):
- LLNL-TR-543366; TRN: US201216%%374
- Country of Publication:
- United States
- Language:
- English
Similar Records
Interrogation of the Burkholderia pseudomallei genome to address differential virulence among isolates
Whole-Genome Sequences of 80 Environmental and Clinical Isolates of Burkholderia pseudomallei