Advances in Assays and Analytical Approaches for Botulinum Toxin Detection

Journal Article · Wed Aug 04 00:00:00 EDT 2010 · Trends in Analytical Chemistry. TrAC, 29(10):1137-1156

DOI:https://doi.org/10.1016/j.trac.2010.07.005· OSTI ID:991572

Grate, Jay W; Ozanich, Richard M; Warner, Marvin G; Bruckner-Lea, Cindy J; Marks, James D

Methods to detect botulinum toxin, the most poisonous substance known, are reviewed. Current assays are being developed with two main objectives in mind: 1) to obtain sufficiently low detection limits to replace the mouse bioassay with an in vitro assay, and 2) to develop rapid assays for screening purposes that are as sensitive as possible while requiring an hour or less to process the sample an obtain the result. This review emphasizes the diverse analytical approaches and devices that have been developed over the last decade, while also briefly reviewing representative older immunoassays to provide background and context.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 991572
• Report Number(s): PNNL-SA-72191; 30400; TRN: US201021%%210
• Journal Information: Trends in Analytical Chemistry. TrAC, 29(10):1137-1156, Vol. 29, Issue 10
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BIOASSAY
DETECTION
IN VITRO
SENSITIVITY
TOXINS
botulinum
toxin
neurotoxin
biothreat
immunoassay
biosensor
flow cytometry
Environmental Molecular Sciences Laboratory