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Title: Clustering of diverse replicated sequences in the MHC: Evidence for en bloc duplication

Abstract

The MHC contains clusters of polymorphic duplicated genes and gene sequences. It has been thought that these duplicated genes and sequences have arisen from single gene duplications. We compared the cloned region between TNF and HLA-B with the region in close proximity to HLA-A using sequence analysis and DNA hybridization. The results indicate that several sequences existing in the region centromeric of HLA-B are also present in close proximity to HLA-A. These include sequences belonging to the P5, BAT1, and PERB11 gene families as well as HLA class I gene sequences. Interestingly, when the two regions of approximately 200 kilobases are compared, the replicated sequences are organized similarly but in an inverted fashion suggesting the existence of an historical inverted en bloc duplication. Thus, we propose that the origin of these MHC gene clusters involves several mechanisms. In addition to single gene replication, a long-range duplication of a genomic block must have occurred. It is possible that a block at the telomeric end of the MHC represents a basic functional genomic unit conserved and duplicated en bloc. 49 refs., 3 figs., 3 tabs.

Authors:
;  [1];  [2]
  1. Univ. Western Australia, Perth (Australia)
  2. Royal Perth Hospital (Australia)
Publication Date:
OSTI Identifier:
99063
Resource Type:
Journal Article
Journal Name:
Journal of Immunology
Additional Journal Information:
Journal Volume: 155; Journal Issue: 2; Other Information: PBD: 15 Jul 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; GENE MUTATIONS; GENE AMPLIFICATION; HISTOCOMPATIBILITY COMPLEX; DNA SEQUENCING; BIOLOGICAL EVOLUTION; CHROMOSOMES; DNA-CLONING; DNA HYBRIDIZATION; ANIMAL CELLS; IMMUNE REACTIONS; CENTROMERES; TELOMERES; HUMAN CHROMOSOME 6; PROBES

Citation Formats

Leelayuwat, C, Pinelli, M, and Dawkins, R L. Clustering of diverse replicated sequences in the MHC: Evidence for en bloc duplication. United States: N. p., 1995. Web.
Leelayuwat, C, Pinelli, M, & Dawkins, R L. Clustering of diverse replicated sequences in the MHC: Evidence for en bloc duplication. United States.
Leelayuwat, C, Pinelli, M, and Dawkins, R L. 1995. "Clustering of diverse replicated sequences in the MHC: Evidence for en bloc duplication". United States.
@article{osti_99063,
title = {Clustering of diverse replicated sequences in the MHC: Evidence for en bloc duplication},
author = {Leelayuwat, C and Pinelli, M and Dawkins, R L},
abstractNote = {The MHC contains clusters of polymorphic duplicated genes and gene sequences. It has been thought that these duplicated genes and sequences have arisen from single gene duplications. We compared the cloned region between TNF and HLA-B with the region in close proximity to HLA-A using sequence analysis and DNA hybridization. The results indicate that several sequences existing in the region centromeric of HLA-B are also present in close proximity to HLA-A. These include sequences belonging to the P5, BAT1, and PERB11 gene families as well as HLA class I gene sequences. Interestingly, when the two regions of approximately 200 kilobases are compared, the replicated sequences are organized similarly but in an inverted fashion suggesting the existence of an historical inverted en bloc duplication. Thus, we propose that the origin of these MHC gene clusters involves several mechanisms. In addition to single gene replication, a long-range duplication of a genomic block must have occurred. It is possible that a block at the telomeric end of the MHC represents a basic functional genomic unit conserved and duplicated en bloc. 49 refs., 3 figs., 3 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/99063}, journal = {Journal of Immunology},
number = 2,
volume = 155,
place = {United States},
year = {Sat Jul 15 00:00:00 EDT 1995},
month = {Sat Jul 15 00:00:00 EDT 1995}
}