Proteomic and Physiological Responses of Kineococcus radiotolerans to Copper
Copper is a highly reactive, toxic metal whose transport into the cell is tightly regulated. Kineococcus radiotolerans was previously shown to specifically accumulate soluble copper in the cytoplasm and cell growth was significantly enhanced by copper during chronic irradiation. This study provides a systematic investigation of copper accumulation, toxicity, and homeostasis in K. radiotolerans through combined physiological experimentation and quantitative shot-gun proteomics. Aerobic growth rates and biomass yields were similar over a range of Cu(II) concentrations, though intracellular metal accumulation was positively correlated with Cu(II) concentration in the growth medium (R2 = 0.7). Global proteomics analysis revealed a significant positive correlation between the total number of response proteins and their abundance with copper concentration and culture age. Approximately 40% of the K. radiotolerans genome was differentially expressed in response to the copper treatments imposed. Copper accumulation coincided with increased abundance of proteins involved in oxidative stress and defense, DNA stabilization and repair, and protein turnover. Concomitant production of antioxidants and protective osmolytes signifies an important adaptation for maintenance of cellular redox; few known metal binding proteins were detected. This study offers a first glimpse into the complexity of coordinated biochemical response pathways in K. radiotolerans invoked by sub-lethal copper concentrations that may be pertinent for new biotechnologies in metal recovery and sequestration, and environmental restoration.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 990116
- Report Number(s):
- PNNL-SA-72375; 17095; KP1704020; TRN: US201020%%127
- Journal Information:
- PLoS One, 5(8):Article No. e12427, Vol. 5, Issue 8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ABUNDANCE
ANTIOXIDANTS
CHRONIC IRRADIATION
COPPER
CYTOPLASM
DNA
DNA REPAIR
HOMEOSTASIS
MAINTENANCE
PRODUCTION
PROTEINS
REPAIR
STABILIZATION
TOXICITY
TRANSPORT
Environmental Molecular Sciences Laboratory