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Title: Perturbations in the Lipid Profile of Individuals with Newly Diagnosed Type 1 Diabetes Mellitus: Lipidomics Analysis of a Diabetes Antibody Standardization Program Sample Subset

Abstract

Objectives: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Design and methods: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. Results: Five hundred sixty lipid features differentiated (q < 0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing of individuals with recently diagnosed type 1 diabetes mellitus. Conclusions: A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization and lipotoxicity of pancreatic beta-cells.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
988647
Report Number(s):
PNNL-SA-70097
Journal ID: ISSN 0009-9120; 12500a; 400412000; TRN: US201018%%601
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Clinical Biochemistry, 43(12):948-956
Additional Journal Information:
Journal Volume: 43; Journal Issue: 12; Journal ID: ISSN 0009-9120
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CHROMATOGRAPHY; DESIGN; DIABETES MELLITUS; ION CYCLOTRON-RESONANCE; LIPIDS; MASS SPECTROMETERS; PLASMA; REGULATIONS; STANDARDIZATION; Environmental Molecular Sciences Laboratory

Citation Formats

Sorensen, Christina M, Ding, Jie, Zhang, Qibin, Alquier, Thierry, Zhao, Rui, Mueller, Patricia W, Smith, Richard D, and Metz, Thomas O. Perturbations in the Lipid Profile of Individuals with Newly Diagnosed Type 1 Diabetes Mellitus: Lipidomics Analysis of a Diabetes Antibody Standardization Program Sample Subset. United States: N. p., 2010. Web. doi:10.1016/j.clinbiochem.2010.04.075.
Sorensen, Christina M, Ding, Jie, Zhang, Qibin, Alquier, Thierry, Zhao, Rui, Mueller, Patricia W, Smith, Richard D, & Metz, Thomas O. Perturbations in the Lipid Profile of Individuals with Newly Diagnosed Type 1 Diabetes Mellitus: Lipidomics Analysis of a Diabetes Antibody Standardization Program Sample Subset. United States. https://doi.org/10.1016/j.clinbiochem.2010.04.075
Sorensen, Christina M, Ding, Jie, Zhang, Qibin, Alquier, Thierry, Zhao, Rui, Mueller, Patricia W, Smith, Richard D, and Metz, Thomas O. 2010. "Perturbations in the Lipid Profile of Individuals with Newly Diagnosed Type 1 Diabetes Mellitus: Lipidomics Analysis of a Diabetes Antibody Standardization Program Sample Subset". United States. https://doi.org/10.1016/j.clinbiochem.2010.04.075.
@article{osti_988647,
title = {Perturbations in the Lipid Profile of Individuals with Newly Diagnosed Type 1 Diabetes Mellitus: Lipidomics Analysis of a Diabetes Antibody Standardization Program Sample Subset},
author = {Sorensen, Christina M and Ding, Jie and Zhang, Qibin and Alquier, Thierry and Zhao, Rui and Mueller, Patricia W and Smith, Richard D and Metz, Thomas O},
abstractNote = {Objectives: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Design and methods: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. Results: Five hundred sixty lipid features differentiated (q < 0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing of individuals with recently diagnosed type 1 diabetes mellitus. Conclusions: A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization and lipotoxicity of pancreatic beta-cells.},
doi = {10.1016/j.clinbiochem.2010.04.075},
url = {https://www.osti.gov/biblio/988647}, journal = {Clinical Biochemistry, 43(12):948-956},
issn = {0009-9120},
number = 12,
volume = 43,
place = {United States},
year = {Sun Aug 01 00:00:00 EDT 2010},
month = {Sun Aug 01 00:00:00 EDT 2010}
}