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Title: Tom, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4

Burkholderia (Pseudomonas) cepacia PR1{sub 23} has been shown to constitutively express a toluene catabolic pathway distinguished by a unique toluene ortho-monooxygenase (Tom). This strain has also been shown to contain two extrachromosomal elements of <70 and> 100 kb. A derivative strain cured of the largest plasmid, PR1{sub 23} Cure, was unable to grow on phenol or toluene as the sole source of carbon and energy, which requires expression of the Tom pathway. Transfer of the larger plasmid from strain G4 J(the parent strain inducible for Tom) enabled PR1{sub 23} Cure to grow on toluene or phenol via inducible Tom pathway expression. Conjugal transfer of TOM{sub 23c} from PR1{sub 23} to an antibiotic-resistant derivative of PR1{sub 23} Cure enabled the transconjugant to grow with either phenol or toluene as the sole source of carbon and energy through constitutive expression of the Tom pathway. A cloned 11.2-kb EcoRI restriction fragment of Tom{sub 23c} resulted in the expression of both Tom and catechol 2,3-dioxygenase in Escherichia coli, as evidenced by its ability to oxidize trichloroethylene, toluene, m-cresol, o-cresol, phenol, and catechol. The largest resident plasmid of PR1 was identified as the source of these genes by DNA hybridization. These results indicate that themore » genes which encode Tom and catechol 2,3-dioxygenase are located on TOM, an approximately 108-kb degradative plasmid of B. cepacia G4. 35 refs., 3 figs., 3 tabs.« less
Authors:
; ;  [1]
  1. Univ. of West Florida, Pensacola, FL (United States) [and others
Publication Date:
OSTI Identifier:
98632
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; Journal Volume: 61; Journal Issue: 4; Other Information: PBD: Apr 1995
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; BIODEGRADATION; GENETIC CONTROL; TOLUENE; PLASMIDS; BIOLOGICAL PATHWAYS; PSEUDOMONAS; CELL CONSTITUENTS