Application of Molecular Techniques to Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site
Abstract
Low-level radioactive waste sites, including those at various U.S. Department of Energy (DOE) sites, frequently contain cellulosic waste in the form of paper towels, cardboard boxes, or wood contaminated with heavy metals and radionuclides such as chromium and uranium. To understand how the soil microbial community is influenced by the presence of cellulosic waste products, multiple soil samples were obtained from a non-radioactive model low-level waste test pit at the Idaho National Laboratory. Samples were analyzed using 16S rDNA clone libraries and 16S rRNA gene microarray (PhyloChip) analyses. Both the clone library and PhyloChip results revealed changes in the bacterial community structure with depth. In all samples, the PhyloChip detected significantly more unique Operational Taxonomic Units (OTUs), and therefore more relative diversity, than the clone libraries. Calculated diversity indices suggest that diversity is lowest in the Fill (F) and Fill Waste (FW) layers and greater in the Wood Waste (WW) and Waste Clay (WC) layers. Principal coordinates analysis and lineage specific analysis determined that Bacteroidetes and Actinobacteria phyla account for most of the significant differences observed between the layers. The decreased diversity in the FW layer and increased members of families containing known cellulose degrading microorganisms suggests the FW layermore »
- Authors:
- Publication Date:
- Research Org.:
- Idaho National Lab. (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- DOE - SC
- OSTI Identifier:
- 983939
- Report Number(s):
- INL/JOU-09-16378
Journal ID: ISSN 0099-2240; TRN: US1005203
- DOE Contract Number:
- DE-AC07-05ID14517
- Resource Type:
- Journal Article
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 76; Journal Issue: 10; Journal ID: ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES/GLOBAL CLIMATE CHANGE STUDIES AND CLIMATE MITIGATION; CELLULOSE; CHROMIUM; CLAYS; GENES; HEAVY METALS; IDAHO; LOW-LEVEL RADIOACTIVE WASTES; MICROORGANISMS; RADIOISOTOPES; SOILS; URANIUM; WASTES; WOOD; WOOD WASTES; cellulose degradation; low level waste; molecular; phylochip
Citation Formats
Field, Erin K, D'Imperio, Seth, Miller, Amber R, VanEngelen, Michael R, Gerlach, Robin, Lee, Brady D, Apel, William A, and Peyton, Brent M. Application of Molecular Techniques to Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site. United States: N. p., 2010.
Web. doi:10.1128/AEM.01688-09.
Field, Erin K, D'Imperio, Seth, Miller, Amber R, VanEngelen, Michael R, Gerlach, Robin, Lee, Brady D, Apel, William A, & Peyton, Brent M. Application of Molecular Techniques to Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site. United States. https://doi.org/10.1128/AEM.01688-09
Field, Erin K, D'Imperio, Seth, Miller, Amber R, VanEngelen, Michael R, Gerlach, Robin, Lee, Brady D, Apel, William A, and Peyton, Brent M. 2010.
"Application of Molecular Techniques to Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site". United States. https://doi.org/10.1128/AEM.01688-09.
@article{osti_983939,
title = {Application of Molecular Techniques to Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site},
author = {Field, Erin K and D'Imperio, Seth and Miller, Amber R and VanEngelen, Michael R and Gerlach, Robin and Lee, Brady D and Apel, William A and Peyton, Brent M},
abstractNote = {Low-level radioactive waste sites, including those at various U.S. Department of Energy (DOE) sites, frequently contain cellulosic waste in the form of paper towels, cardboard boxes, or wood contaminated with heavy metals and radionuclides such as chromium and uranium. To understand how the soil microbial community is influenced by the presence of cellulosic waste products, multiple soil samples were obtained from a non-radioactive model low-level waste test pit at the Idaho National Laboratory. Samples were analyzed using 16S rDNA clone libraries and 16S rRNA gene microarray (PhyloChip) analyses. Both the clone library and PhyloChip results revealed changes in the bacterial community structure with depth. In all samples, the PhyloChip detected significantly more unique Operational Taxonomic Units (OTUs), and therefore more relative diversity, than the clone libraries. Calculated diversity indices suggest that diversity is lowest in the Fill (F) and Fill Waste (FW) layers and greater in the Wood Waste (WW) and Waste Clay (WC) layers. Principal coordinates analysis and lineage specific analysis determined that Bacteroidetes and Actinobacteria phyla account for most of the significant differences observed between the layers. The decreased diversity in the FW layer and increased members of families containing known cellulose degrading microorganisms suggests the FW layer is an enrichment environment for cellulose degradation. Overall, these results suggest that the presence of the cellulosic material significantly influences the bacterial community structure in a stratified soil system.},
doi = {10.1128/AEM.01688-09},
url = {https://www.osti.gov/biblio/983939},
journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 10,
volume = 76,
place = {United States},
year = {Sat May 01 00:00:00 EDT 2010},
month = {Sat May 01 00:00:00 EDT 2010}
}