Salmonella Enterica Serovar Typhimurium BipA Exhibits Two Distinct Ribosome Binding Modes

Journal Article · Tue Jan 01 00:00:00 EST 2008 · Journal of Bacteriology

DOI:https://doi.org/10.1128/JB.00763-08· OSTI ID:980442

deLivron, M; Robinson, V

BipA is a highly conserved prokaryotic GTPase that functions to influence numerous cellular processes in bacteria. In Escherichia coli and Salmonella enterica serovar Typhimurium, BipA has been implicated in controlling bacterial motility, modulating attachment and effacement processes, and upregulating the expression of virulence genes and is also responsible for avoidance of host defense mechanisms. In addition, BipA is thought to be involved in bacterial stress responses, such as those associated with virulence, temperature, and symbiosis. Thus, BipA is necessary for securing bacterial survival and successful invasion of the host. Steady-state kinetic analysis and pelleting assays were used to assess the GTPase and ribosome-binding properties of S. enterica BipA. Under normal bacterial growth, BipA associates with the ribosome in the GTP-bound state. However, using sucrose density gradients, we demonstrate that the association of BipA and the ribosome is altered under stress conditions in bacteria similar to those experienced during virulence. The data show that this differential binding is brought about by the presence of ppGpp, an alarmone that signals the onset of stress-related events in bacteria.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: DE-AC02-98CH10886
• OSTI ID: 980442
• Report Number(s): BNL-93360-2010-JA; JOBAAY; TRN: US201015%%1827
• Journal Information: Journal of Bacteriology, Vol. 190, Issue 17; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
AVOIDANCE
BACTERIA
DATA
DENSITY
ESCHERICHIA COLI
FUNCTIONS
GENES
GROWTH
HOST
KINETICS
NATIONAL DEFENSE
RIBOSOMES
SACCHAROSE
SALMONELLA
SIGNALS
SYMBIOSIS
VIRULENCE
national synchrotron light source