Phenotype screening for genetically deermined age-onset disorders and increased longevity in ENU-mutagenized mice

Journal Article · Sat Jan 01 00:00:00 EST 2005 · Age (Omaha)

DOI:https://doi.org/10.1007/s11357-005-4131-3· OSTI ID:978201

Johnson, Dabney K ^[1]; Rinchik, Eugene M ^[1]; Moustaid-Moussa, Naima ^[2]; Miller, Darla R ^[1]; Williams, Robert ^[3]; Michaud, III, Edward J ^[1]; Jablonski, Monica M. ^[3]; Elberger, Andrea ^[3]; Hamre, Kristin M. ^[3]; Smeyne, Richard ^[4]; Chesler, Elissa J ^[1]; Goldowitz, Daniel ^[3]

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1. ORNL
2. University of Tennessee, Knoxville (UTK)
3. University of Tennessee Health Science Center, Memphis
4. St. Jude Children's Research Hospital

With the goal of discovering genes that contribute to late-onset neurological and ocular disorders and also genes that extend the healthy life span in mammals, we are phenotyping mice carrying new mutations induced by the chemical N-ethyl-N-nitrosourea (ENU). The phenotyping plan includes basic behavioral, neurohistological, and vision testing in sibling cohorts of mice aged to 18 months, and then evaluation for markers of growth trajectory and stress response in these same cohorts aged up to 28 months. Statistical outliers are identified by comparison to test results of similar aged cohorts, and potential mutants are recovered for re-aging to confirm heritability of the phenotype.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mouse Genetics Research Facility
• Sponsoring Organization: USDOE; Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 978201
• Journal Information: Age (Omaha), Vol. 27, Issue 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
EVALUATION
GENES
LIFE SPAN
MAMMALS
MICE
MUTANTS
MUTATIONS
PHENOTYPE
TESTING
age-onset
ENU mutations
longevity
neurological disorders
pheontype-screening