Mapping protein abundance patterns in the brain using voxelation combined with liquid chromatography and mass spectrometry

Journal Article · Mon Feb 01 00:00:00 EST 2010 · Methods, 50(2):77-84

DOI:https://doi.org/10.1016/j.ymeth.2009.07.009· OSTI ID:974933

Petyuk, Vladislav A; Qian, Weijun; Smith, Richard D; Smith, Desmond J

Voxelation creates expression atlases by high-throughput analysis of spatially registered cubes or voxels harvested from the brain. The modality independence of voxelation allows a variety of bioanalytical techniques to be used to map abundance. Protein expression patterns in the brain can be obtained using liquid chromatography (LC) combined with mass spectrometry (MS). Here we describe the methodology of voxelation as it pertains particularly to LC-MS proteomic analysis: sample preparation, instrumental set up and analysis, peptide identification and protein relative abundance quantitation. We also briefly describe some of the advantages, limitations and insights into the brain that can be obtained using combined proteomic and transcriptomic maps

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 974933
• Report Number(s): PNNL-SA-68829; 24698; 20496; 400412000; TRN: US201007%%892
• Journal Information: Methods, 50(2):77-84, Vol. 50, Issue 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ABUNDANCE
BRAIN
CHROMATOGRAPHY
MASS SPECTROSCOPY
PEPTIDES
PROTEINS
SAMPLE PREPARATION
Environmental Molecular Sciences Laboratory