Differentiation of Gram-Negative Bacterial Aerosol Exposure Using Detected Markers in Bronchial-Alveolar Lavage Fluid

Journal Article · Wed Sep 16 00:00:00 EDT 2009 · PLoS One, 4(9):e7047

DOI:https://doi.org/10.1371/journal.pone.0007047· OSTI ID:973998

Wunschel, David S; Webb-Robertson, Bobbie-Jo M; Frevert, Charles W; Skerret, Shawn J; Beagley, Nathaniel; Willse, Alan R; Colburn, Heather A; Antolick, Kathryn C

The identification of biosignatures of aerosol exposure to pathogens has the potential to provide useful diagnostic information. In particular, markers of exposure to different types of respiratory pathogens may yield diverse sets of markers that can be used to differentiate exposure. We examine a of mouse model of aerosol exposure to known Gram negative bacterial pathogens, Francisella tularensis novicida and Pseudomonas auriginosa. Mice were subjected to either a pathogen or control exposure and bronchial alveolar lavage fluid (BALF) was collected at four and twenty four hours post exposure. Small protein and peptide markers within the BALF were detected by matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) and analyzed using both exploratory and predictive data analysis methods; principle component analysis and degree of association. The markers detected were successfully used to accurately identify the four hour exposed samples from the control samples. This report demonstrates the potential for small protein and peptide marker profiles to identify aerosol exposure in a short post-exposure time frame.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 973998
• Report Number(s): PNNL-SA-64283; TRN: US201007%%175
• Journal Information: PLoS One, 4(9):e7047, Vol. 4, Issue 9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
AEROSOLS
DATA ANALYSIS
LASERS
LAVAGE
MASS SPECTROSCOPY
MICE
PATHOGENS
PEPTIDES
PROTEINS
PSEUDOMONAS
Peptide biomarkers
Gram negative aerosol exposure
Matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)
Principle component analysis (PCA)