Chemometric Analysis of Multiple Species of Bacillus Bacterial Endospores Using Infrared Spectroscopy: Discrimination to the Strain Level
Previous work using infrared spectroscopy has shown potential for rapid discrimination between bacteria in either their sporulated or vegetative states, as well as between bacteria and other common interferents. For species within one physiological state, however, distinction is far more challenging, and requires chemometrics. In the current study, we have narrowed the field of study by eliminating the confounding issues of vegetative cells as well as growth media and focused on using IR spectra to distinguish between different species all in the sporulated state. Using principal component analysis (PCA) and a classification method based upon similarity measurements, we demonstrate a successful identification rate to the species level of 85% for Bacillus spores grown and sporulated in a glucose broth medium.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 973711
- Report Number(s):
- PNNL-SA-66451; ACACAM; NN2001000; TRN: US201006%%1075
- Journal Information:
- Analytica Chimica Acta, 651(1):24-30, Vol. 651, Issue 1; ISSN 0003-2670
- Country of Publication:
- United States
- Language:
- English
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