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Title: Protein subcellular localization assays using split fluorescent proteins

The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).
Authors:
 [1] ;  [2]
  1. Santa Fe, NM
  2. Los Alamos, NM
Publication Date:
OSTI Identifier:
971623
Report Number(s):
7,585,636
US patent application 11/295,374
DOE Contract Number:
W-7405-ENG-36
Resource Type:
Patent
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Sponsoring Org:
United States Department of Energy
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES