Phenotype Microarray Profiling of Zymomonas mobilis ZM4

Journal Article · Thu Jan 01 00:00:00 EST 2009 · Applied Biochemistry and Biotechnology

OSTI ID:969774

Bochner, Barry ^[1]; Gomez, Vanessa ^[1]; Ziman, michael ^[1]; Yang, Shihui ^[2]; Brown, Steven D ^[2]

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1. Biolog, Inc.
2. ORNL

In this study, we developed a Phenotype MicroArray{trademark} (PM) protocol to profile cellular phenotypes in Zymomonas mobilis, which included a standard set of nearly 2,000 assays for carbon, nitrogen, phosphorus and sulfur source utilization, nutrient stimulation, pH and osmotic stresses, and chemical sensitivities with 240 inhibitory chemicals. We observed two positive assays for C-source utilization (fructose and glucose) using the PM screen, which uses redox chemistry and cell respiration as a universal reporter to profile growth phenotypes in a high-throughput 96-well plate-based format. For nitrogen metabolism, the bacterium showed a positive test results for ammonia, aspartate, asparagine, glutamate, glutamine, and peptides. Z. mobilis appeared to use a diverse array of P-sources with two exceptions being pyrophosphate and tripolyphosphate. The assays suggested that Z. mobilis uses both inorganic and organic compounds as S-sources. No stimulation by nutrients was detected; however, there was evidence of partial inhibition by purines and pyrimidines, NAD, and deferoxamine. Z. mobilis was relatively resistant to acid pH, tolerating a pH down to about 4.0. It also tolerated phosphate, sulfate, and nitrate, but was rather sensitive to chloride and nitrite. Z. mobilis showed resistance to a large number of diverse chemicals that inhibit most bacteria. The information from PM analysis provides an overview of Z. mobilis physiology and a foundation for future comparisons of other wild-type and mutant Z. mobilis strains.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 969774
• Journal Information: Applied Biochemistry and Biotechnology, Vol. eprint, Issue 1-8; ISSN 0273-2289
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
AMMONIA
ASPARAGINE
CARBON
CHLORIDES
DEFEROXAMINE
GLUTAMINE
METABOLISM
MUTANTS
NITROGEN
ORGANIC COMPOUNDS
PEPTIDES
PHENOTYPE
PHOSPHORUS
PURINES
PYRIMIDINES
PYROPHOSPHATES
STIMULATION
SULFUR
ZYMOMONAS MOBILIS
Ethanol
fermentation
physiology
systems biology
LDRD
BESC
Phenotype
Microarray
Zymomonas