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Title: Can Delignification Decrease Cellulose Digestibility in Acid Pretreated Corn Stover?

Abstract

It has previously been shown that the improved digestibility of dilute acid pretreated corn stover is at least partially due to the removal of xylan and the consequent increase in accessibility of the cellulose to cellobiohydrolase enzymes. We now report on the impact that lignin removal has on the accessibility and digestibility of dilute acid pretreated corn stover. Samples of corn stover were subjected to dilute sulfuric acid pretreatment with and without simultaneous (partial) lignin removal. In addition, some samples were completely delignified after the pretreatment step using acidified sodium chlorite. The accessibility and digestibility of the samples were tested using a fluorescence-labeled cellobiohydrolase (Trichoderma reesei Cel7A) purified from a commercial cellulase preparation. Partial delignification of corn stover during dilute acid pretreatment was shown to improve cellulose digestibility by T. reesei Cel7A; however, decreasing the lignin content below 5% (g g{sup -1}) by treatment with acidified sodium chlorite resulted in a dramatic reduction in cellulose digestibility. Importantly, this effect was found to be enhanced in samples with lower xylan contents suggesting that the near complete removal of xylan and lignin may cause aggregation of the cellulose microfibrils resulting in decreased cellulase accessibility.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
968194
DOE Contract Number:  
AC36-99-GO10337
Resource Type:
Journal Article
Journal Name:
Cellulose
Additional Journal Information:
Journal Volume: 16; Journal Issue: 4, 2009
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; AGRICULTURAL WASTES; CELLULASE; CELLULOSE; CHLORINE COMPOUNDS; DELIGNIFICATION; ENZYMES; LIGNIN; MAIZE; OXYGEN COMPOUNDS; REMOVAL; SODIUM; SULFURIC ACID; TRICHODERMA; XYLANS; Chemical and Biosciences

Citation Formats

Ishizawa, C I, Jeoh, T, Adney, W S, Himmel, M E, Johnson, D K, and Davis, M F. Can Delignification Decrease Cellulose Digestibility in Acid Pretreated Corn Stover?. United States: N. p., 2009. Web. doi:10.1007/s10570-009-9313-1.
Ishizawa, C I, Jeoh, T, Adney, W S, Himmel, M E, Johnson, D K, & Davis, M F. Can Delignification Decrease Cellulose Digestibility in Acid Pretreated Corn Stover?. United States. https://doi.org/10.1007/s10570-009-9313-1
Ishizawa, C I, Jeoh, T, Adney, W S, Himmel, M E, Johnson, D K, and Davis, M F. 2009. "Can Delignification Decrease Cellulose Digestibility in Acid Pretreated Corn Stover?". United States. https://doi.org/10.1007/s10570-009-9313-1.
@article{osti_968194,
title = {Can Delignification Decrease Cellulose Digestibility in Acid Pretreated Corn Stover?},
author = {Ishizawa, C I and Jeoh, T and Adney, W S and Himmel, M E and Johnson, D K and Davis, M F},
abstractNote = {It has previously been shown that the improved digestibility of dilute acid pretreated corn stover is at least partially due to the removal of xylan and the consequent increase in accessibility of the cellulose to cellobiohydrolase enzymes. We now report on the impact that lignin removal has on the accessibility and digestibility of dilute acid pretreated corn stover. Samples of corn stover were subjected to dilute sulfuric acid pretreatment with and without simultaneous (partial) lignin removal. In addition, some samples were completely delignified after the pretreatment step using acidified sodium chlorite. The accessibility and digestibility of the samples were tested using a fluorescence-labeled cellobiohydrolase (Trichoderma reesei Cel7A) purified from a commercial cellulase preparation. Partial delignification of corn stover during dilute acid pretreatment was shown to improve cellulose digestibility by T. reesei Cel7A; however, decreasing the lignin content below 5% (g g{sup -1}) by treatment with acidified sodium chlorite resulted in a dramatic reduction in cellulose digestibility. Importantly, this effect was found to be enhanced in samples with lower xylan contents suggesting that the near complete removal of xylan and lignin may cause aggregation of the cellulose microfibrils resulting in decreased cellulase accessibility.},
doi = {10.1007/s10570-009-9313-1},
url = {https://www.osti.gov/biblio/968194}, journal = {Cellulose},
number = 4, 2009,
volume = 16,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2009},
month = {Thu Jan 01 00:00:00 EST 2009}
}