skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Fast atom bombardment tandem mass spectrometry of carotenoids

Abstract

Positive ion fast atom bombardment (FAB) tandem mass spectrometry (MS-MS) using a double-focusing mass spectrometer with linked scanning at constant B/E and high-energy collisionally activated dissociation (CAD) was used to differentiate 17 different cartenoids, including {beta}-apo-8{prime}- carotenal, astaxanthin, {alpha}-carotene, {beta}-carotene, {gamma}-carotene, {zeta}-carotene, canthaxanthin, {beta}-cryptoxanthin, isozeaxanthin bis (pelargonate), neoxanthin, neurosporene, nonaprene, lutein, lycopene, phytoene, phytofluene, and zeaxanthin. The carotenoids were either synthetic or isolated from plant tissues. The use of FAB ionization minimized degradation or rearrangement of the carotenoid structures due to the inherent thermal instability generally ascribed to these compounds. Instead of protonated molecules, both polar xanthophylls and nonpolar carotenes formed molecular ions, M{sup {center_dot}+}, during FAB ionization. Following collisionally activated dissociation, fragment ions of selected molecular ion precursors showed structural features indicative of the presence of hydroxyl groups, ring systems, ester groups, and aldehyde groups and the extent of aliphatic polyene conjugation. The fragmentation patterns observed in the mass spectra herein may be used as a reference for the structural determination of carotenoids isolated from plant and animal tissues. 18 refs., 4 figs.

Authors:
 [1]; ;  [2]
  1. Univ. of Illinois, Chicago, IL (United States)
  2. North Carolina State Univ., Raleigh, NC (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
96379
Resource Type:
Journal Article
Journal Name:
Journal of Agricultural and Food Chemistry
Additional Journal Information:
Journal Volume: 43; Journal Issue: 2; Other Information: PBD: Feb 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; CAROTENOIDS; IONIZATION; RADIOCHEMICAL ANALYSIS; ANIMAL TISSUES; FRAGMENTATION; MOLECULAR IONS; PLANT TISSUES; MASS SPECTROSCOPY

Citation Formats

van Breeman, R B, Schmitz, H H, and Schwartz, S J. Fast atom bombardment tandem mass spectrometry of carotenoids. United States: N. p., 1995. Web. doi:10.1021/jf00050a024.
van Breeman, R B, Schmitz, H H, & Schwartz, S J. Fast atom bombardment tandem mass spectrometry of carotenoids. United States. https://doi.org/10.1021/jf00050a024
van Breeman, R B, Schmitz, H H, and Schwartz, S J. 1995. "Fast atom bombardment tandem mass spectrometry of carotenoids". United States. https://doi.org/10.1021/jf00050a024.
@article{osti_96379,
title = {Fast atom bombardment tandem mass spectrometry of carotenoids},
author = {van Breeman, R B and Schmitz, H H and Schwartz, S J},
abstractNote = {Positive ion fast atom bombardment (FAB) tandem mass spectrometry (MS-MS) using a double-focusing mass spectrometer with linked scanning at constant B/E and high-energy collisionally activated dissociation (CAD) was used to differentiate 17 different cartenoids, including {beta}-apo-8{prime}- carotenal, astaxanthin, {alpha}-carotene, {beta}-carotene, {gamma}-carotene, {zeta}-carotene, canthaxanthin, {beta}-cryptoxanthin, isozeaxanthin bis (pelargonate), neoxanthin, neurosporene, nonaprene, lutein, lycopene, phytoene, phytofluene, and zeaxanthin. The carotenoids were either synthetic or isolated from plant tissues. The use of FAB ionization minimized degradation or rearrangement of the carotenoid structures due to the inherent thermal instability generally ascribed to these compounds. Instead of protonated molecules, both polar xanthophylls and nonpolar carotenes formed molecular ions, M{sup {center_dot}+}, during FAB ionization. Following collisionally activated dissociation, fragment ions of selected molecular ion precursors showed structural features indicative of the presence of hydroxyl groups, ring systems, ester groups, and aldehyde groups and the extent of aliphatic polyene conjugation. The fragmentation patterns observed in the mass spectra herein may be used as a reference for the structural determination of carotenoids isolated from plant and animal tissues. 18 refs., 4 figs.},
doi = {10.1021/jf00050a024},
url = {https://www.osti.gov/biblio/96379}, journal = {Journal of Agricultural and Food Chemistry},
number = 2,
volume = 43,
place = {United States},
year = {Wed Feb 01 00:00:00 EST 1995},
month = {Wed Feb 01 00:00:00 EST 1995}
}