Identification of a New Locus, Ptr(t), Required for Rice Blast Resistance Gene Pi-ta-Mediated Resistance
- ORNL
Resistance to the blast pathogen Magnaporthe oryzae is proposed to be initiated by physical binding of a putative cytoplasmic receptor encoded by a NBS type resistance gene Pi-ta to the processed elicitor encoded by the corresponding avirulence gene AVR-Pita. Here we report the identification of a new locus Ptr(t) that is required for Pi-ta-mediated signal recognition. A Pi-ta expressing susceptible mutant was identified using a genetic screen. Putative mutations at Ptr(t) does not alter recognition specificity to another resistance gene Pi-ks in the Pi-ta homozygote indicate that Ptr(t) is more likely specific to Pi-ta-mediated signal recognition. Genetic crosses of Pi-ta Ptr(t) and Pi-ta ptr(t) homozygotes suggest that Ptr(t) segregate at single dominant nuclear gene. A ratio of 1 resistant: 1 susceptible of a BC1 using Pi-ta Ptr(t) with pi-ta ptr(t) homozygotes indicates that Pi-ta and Ptr(t) are linked and co-segregated. Genotyping of mutants of pi-ta ptr(t) and Pi-ta Ptr(t) homozygotes using ten simple sequence repeat markers spanning 9 megabase of Pi-ta determines that Pi-ta and Ptr(t) are of indica origin. Identification of Ptr(t) is a significant advancement in studying Pi-ta-mediated signal recognition and transduction.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Californium User Facility for Neutron Science
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- DE-AC05-00OR22725
- OSTI ID:
- 963377
- Journal Information:
- Molecular Plant-Microbe Interactions, Vol. 21, Issue 4
- Country of Publication:
- United States
- Language:
- English
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