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Title: Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks

Abstract

When Chinese hamster ovary cells were treated with ultraviolet (UV) light or methyl methane-sulfonate (MMS), a large number of DNA strand breaks could be detected by alkaline elution. These strand breaks gradually disappeared if the treated cells were allowed to recover in a drug-free medium. The presence of nickel or arsenite during the recovery incubation retarded the disappearance of UV-induced strand breaks, whereas the disappearance of MMS-induced strand breaks was retarded by the presence of arsenite or of luminol, a new inhibit for poly(ADP-ribose) synthetase. Luminol, however, had no apparent effect on the repair of UV-induced DNA strand breaks, and nickel had no effect on the repair of MMS-induced DNA strand breaks. When UV- or MMS-treated cells were incubated in cytosine arabinofuranoside (AraC) plus hydroxyurea (HU), a large amount of low molecular weight DNA was detected by alkaline sucrose sedimentation. The molecular weight of these DNAs increased if the cells were further incubated in a drug-free medium. This rejoining of breaks in cells pretreated with UV plus AraC and HU was inhibited by nickel and by arsenite, but not by luminol. The rejoining of breaks in cells pretreated with MMS plus AraC and HU was inhibited by luminol and bymore » arsenite, but not by nickel. These results suggest that different enzymes may be used in DNA resynthesis and/or ligation during the repairing of UV- and MMS-induced DNA strand breaks, and that nickel, luminol, and arsenite may have differential inhibitory effects on these enzymes. 29 refs., 4 figs., 1 tab.« less

Authors:
; ;  [1]
  1. Institute of Zoology, Taipei (Taiwan, Province of China); and others
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
96311
Resource Type:
Journal Article
Journal Name:
Environmental and Molecular Mutagenesis
Additional Journal Information:
Journal Volume: 23; Journal Issue: 2; Other Information: PBD: 1994
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CHO CELLS; DNA REPAIR; RESPONSE MODIFYING FACTORS; NICKEL; LUMINOL; BIOLOGICAL PATHWAYS; ARSENIC; ENZYMES; STRAND BREAKS; BIOLOGICAL EFFECTS

Citation Formats

Lee-Chen, S F, Yu, C T, and Wu, D R. Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks. United States: N. p., 1994. Web. doi:10.1002/em.2850230207.
Lee-Chen, S F, Yu, C T, & Wu, D R. Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks. United States. https://doi.org/10.1002/em.2850230207
Lee-Chen, S F, Yu, C T, and Wu, D R. 1994. "Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks". United States. https://doi.org/10.1002/em.2850230207.
@article{osti_96311,
title = {Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks},
author = {Lee-Chen, S F and Yu, C T and Wu, D R},
abstractNote = {When Chinese hamster ovary cells were treated with ultraviolet (UV) light or methyl methane-sulfonate (MMS), a large number of DNA strand breaks could be detected by alkaline elution. These strand breaks gradually disappeared if the treated cells were allowed to recover in a drug-free medium. The presence of nickel or arsenite during the recovery incubation retarded the disappearance of UV-induced strand breaks, whereas the disappearance of MMS-induced strand breaks was retarded by the presence of arsenite or of luminol, a new inhibit for poly(ADP-ribose) synthetase. Luminol, however, had no apparent effect on the repair of UV-induced DNA strand breaks, and nickel had no effect on the repair of MMS-induced DNA strand breaks. When UV- or MMS-treated cells were incubated in cytosine arabinofuranoside (AraC) plus hydroxyurea (HU), a large amount of low molecular weight DNA was detected by alkaline sucrose sedimentation. The molecular weight of these DNAs increased if the cells were further incubated in a drug-free medium. This rejoining of breaks in cells pretreated with UV plus AraC and HU was inhibited by nickel and by arsenite, but not by luminol. The rejoining of breaks in cells pretreated with MMS plus AraC and HU was inhibited by luminol and by arsenite, but not by nickel. These results suggest that different enzymes may be used in DNA resynthesis and/or ligation during the repairing of UV- and MMS-induced DNA strand breaks, and that nickel, luminol, and arsenite may have differential inhibitory effects on these enzymes. 29 refs., 4 figs., 1 tab.},
doi = {10.1002/em.2850230207},
url = {https://www.osti.gov/biblio/96311}, journal = {Environmental and Molecular Mutagenesis},
number = 2,
volume = 23,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}