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Title: A renaissance for the pioneering 16S rRNA gene

Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the last quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community's functional potential rather than just an inventory of its inhabitants. However, the pioneering 16S rRNA gene is making a comeback in its own right thanks to a number of methodological advancements including higher resolution (more sequences), analysis of multiple related samples (e.g. spatial and temporal series) and improved metadata and use of metadata. The standard conclusion that microbial ecosystems are remarkably complex and diverse is now being replaced by detailed insights into microbial ecology and evolution based only on this one historically important marker gene.
Authors:
;
Publication Date:
OSTI Identifier:
962659
Report Number(s):
LBNL-2015E
Journal ID: ISSN 1369-5274; TRN: US200916%%305
DOE Contract Number:
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Current Opinion in Microbiology; Journal Volume: 11; Journal Issue: 5; Related Information: Journal Publication Date: October 2008
Research Org:
Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
Sponsoring Org:
Genomics Division
Country of Publication:
United States
Language:
English
Subject:
59; ECOLOGY; ECOSYSTEMS; FUNCTIONALS; GENES; RESOLUTION Bacteria/classification, Bacteria/genetics, Biodiversity, Cluster Analysis, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, RNA, Ribosomal, 16S/genetics