Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens
Abstract
The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation due to its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of your antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 957360
- Report Number(s):
- PNNL-SA-53301
400412000; TRN: US201007%%667
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Book
- Resource Relation:
- Related Information: Biomarker Methods in Drug Discovery and Development, 273-290
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ENZYME IMMUNOASSAY; PROBES; PROCESSING; PROTEINS; SCREENS; VALIDATION; Antibody microarrays; sandwich ELISA,; proteomics; high throughput protein assays; biomarker discovery
Citation Formats
Gonzalez, Rachel M, Varnum, Susan M, and Zangar, Richard C. Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens. United States: N. p., 2009.
Web.
Gonzalez, Rachel M, Varnum, Susan M, & Zangar, Richard C. Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens. United States.
Gonzalez, Rachel M, Varnum, Susan M, and Zangar, Richard C. 2009.
"Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens". United States.
@article{osti_957360,
title = {Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens},
author = {Gonzalez, Rachel M and Varnum, Susan M and Zangar, Richard C},
abstractNote = {The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation due to its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of your antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.},
doi = {},
url = {https://www.osti.gov/biblio/957360},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon May 11 00:00:00 EDT 2009},
month = {Mon May 11 00:00:00 EDT 2009}
}
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