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Title: Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens

Abstract

The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation due to its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of your antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
957360
Report Number(s):
PNNL-SA-53301
400412000; TRN: US201007%%667
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Book
Resource Relation:
Related Information: Biomarker Methods in Drug Discovery and Development, 273-290
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ENZYME IMMUNOASSAY; PROBES; PROCESSING; PROTEINS; SCREENS; VALIDATION; Antibody microarrays; sandwich ELISA,; proteomics; high throughput protein assays; biomarker discovery

Citation Formats

Gonzalez, Rachel M, Varnum, Susan M, and Zangar, Richard C. Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens. United States: N. p., 2009. Web.
Gonzalez, Rachel M, Varnum, Susan M, & Zangar, Richard C. Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens. United States.
Gonzalez, Rachel M, Varnum, Susan M, and Zangar, Richard C. 2009. "Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens". United States.
@article{osti_957360,
title = {Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens},
author = {Gonzalez, Rachel M and Varnum, Susan M and Zangar, Richard C},
abstractNote = {The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation due to its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of your antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.},
doi = {},
url = {https://www.osti.gov/biblio/957360}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon May 11 00:00:00 EDT 2009},
month = {Mon May 11 00:00:00 EDT 2009}
}

Book:
Other availability
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