Small Molecule-Induced Allosteric Activation of the Vibrio Cholerae RTX Cysteine Protease Domain
Journal Article
·
· Science 322:265,2008
OSTI ID:953100
Vibrio cholerae RTX (repeats in toxin) is an actin-disrupting toxin that is autoprocessed by an internal cysteine protease domain (CPD). The RTX CPD is efficiently activated by the eukaryote-specific small molecule inositol hexakisphosphate (InsP{sub 6}), and we present the 2.1 angstrom structure of the RTX CPD in complex with InsP{sub 6}. InsP{sub 6} binds to a conserved basic cleft that is distant from the protease active site. Biochemical and kinetic analyses of CPD mutants indicate that InsP{sub 6} binding induces an allosteric switch that leads to the autoprocessing and intracellular release of toxin-effector domains.
- Research Organization:
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC02-76SF00515
- OSTI ID:
- 953100
- Report Number(s):
- SLAC-REPRINT-2009-184; SCEHDK; TRN: US200914%%196
- Journal Information:
- Science 322:265,2008, Vol. 322; ISSN 0193-4511
- Country of Publication:
- United States
- Language:
- English
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