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Title: Encapsulation of Semiconducting Polymers in Vault Protein Cages

Abstract

We demonstrate that a semiconducting polymer [poly(2-methoxy-5-propyloxy sulfonate phenylene vinylene), MPS-PPV] can be encapsulated inside recombinant, self-assembling protein nanocapsules called 'vaults'. Polymer incorporation into these nanosized protein cages, found naturally at {approx}10,000 copies per human cell, was confirmed by fluorescence spectroscopy and small-angle X-ray scattering. Although vault cellular functions and gating mechanisms remain unknown, their large internal volume and natural prevalence within the human body suggests they could be used as carriers for therapeutics and medical imaging reagents. This study provides the groundwork for the use of vaults in encapsulation and delivery applications.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
953027
Report Number(s):
SLAC-REPRINT-2009-257
TRN: US200914%%260
DOE Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article
Journal Name:
Nano Lett 8:3503,2008
Additional Journal Information:
Journal Volume: 8; Journal Issue: 10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; ENCAPSULATION; FLUORESCENCE SPECTROSCOPY; POLYMERS; PROTEINS; SCATTERING; SULFONATES; Other,OTHER

Citation Formats

Ng, B C, Yu, M, Gopal, A, Rome, L H, Monbouquette, H G, and Tolbert, S H. Encapsulation of Semiconducting Polymers in Vault Protein Cages. United States: N. p., 2009. Web.
Ng, B C, Yu, M, Gopal, A, Rome, L H, Monbouquette, H G, & Tolbert, S H. Encapsulation of Semiconducting Polymers in Vault Protein Cages. United States.
Ng, B C, Yu, M, Gopal, A, Rome, L H, Monbouquette, H G, and Tolbert, S H. 2009. "Encapsulation of Semiconducting Polymers in Vault Protein Cages". United States.
@article{osti_953027,
title = {Encapsulation of Semiconducting Polymers in Vault Protein Cages},
author = {Ng, B C and Yu, M and Gopal, A and Rome, L H and Monbouquette, H G and Tolbert, S H},
abstractNote = {We demonstrate that a semiconducting polymer [poly(2-methoxy-5-propyloxy sulfonate phenylene vinylene), MPS-PPV] can be encapsulated inside recombinant, self-assembling protein nanocapsules called 'vaults'. Polymer incorporation into these nanosized protein cages, found naturally at {approx}10,000 copies per human cell, was confirmed by fluorescence spectroscopy and small-angle X-ray scattering. Although vault cellular functions and gating mechanisms remain unknown, their large internal volume and natural prevalence within the human body suggests they could be used as carriers for therapeutics and medical imaging reagents. This study provides the groundwork for the use of vaults in encapsulation and delivery applications.},
doi = {},
url = {https://www.osti.gov/biblio/953027}, journal = {Nano Lett 8:3503,2008},
number = 10,
volume = 8,
place = {United States},
year = {Fri May 22 00:00:00 EDT 2009},
month = {Fri May 22 00:00:00 EDT 2009}
}