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Title: Cell-Cell Interactions during pollen tube guidance

Technical Report ·
OSTI ID:950456

The long-term goal of this research is to identify the signaling molecules that mediate plant cell-cell interactions during pollination. The immediate goals of this project are to perform genetic and molecular analysis of pollen tube guidance. Specifically, we proposed to: 1. Characterize the pistil components that direct pollen tube navigation using the Arabidopsis thaliana in vitro pollen tube guidance system 2. Identify pistil signals that direct pollen tube guidance by a) using microarrays to profile gene expression in developing pistils, and b) employing proteomics and metabolomics to isolate pollen tube guidance signals. 3. Explore the genetic basis of natural variation in guidance signals, comparing the in vitro interactions between pollen and pistils from A. thaliana and its close relatives.

Research Organization:
The University of Chicago, Chicago, IL
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
FG02-96ER20240
OSTI ID:
950456
Report Number(s):
DOE/ER20240-Final Report
Country of Publication:
United States
Language:
English

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