Express primer tool for high-throughput gene cloning and expression.
Journal Article
·
· Biotech.
OSTI ID:949669
High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- National Institutes of Health (NIH)
- DOE Contract Number:
- DE-AC02-06CH11357
- OSTI ID:
- 949669
- Report Number(s):
- ANL/BIO/JA-43150; BTGYDD; TRN: US201012%%449
- Journal Information:
- Biotech., Vol. 33, Issue 6 ; Dec. 2002; ISSN 0740-7378
- Country of Publication:
- United States
- Language:
- ENGLISH
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