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Title: Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-containing Surface Scaffolds

Abstract

We describe the successful development of a procedure for the step-by-step formation of a reactive, multi-layer polymer scaffold incorporating polymers based on 2-vinyl-4,4-dimethylazlactone (VDMA) on a silicon wafer and the characterization of these materials. Also discussed is the development of a procedure for the non-site specific attachment of a biomolecule to the modified silicon wafer, including scaffolds modified via drop-on-demand (DOD) inkjet printing. VDMA-based polymers were used because of their hydrolytic stability and ability of the pendant azlactone rings to form stable covalent bonds with primary amines without byproducts via nucleophilic addition. This reaction proceeds without a catalyst and at room temperature, yielding a stable amide linkage, which adds to the ease of construction expected when using VDMA-based polymers. DOD inkjet printing was explored as an interesting method for creating surfaces with one or more patterns of biomolecules, because of the flexibility and ease in pattern design.

Authors:
 [1];  [2];  [1];  [2]
  1. Clemson University
  2. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
948853
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Langmuir
Additional Journal Information:
Journal Volume: 25; Journal Issue: 1; Journal ID: ISSN 0743-7463
Country of Publication:
United States
Language:
English

Citation Formats

Barrninger, Joshua, Messman, Jamie M, Banaszak, Abigail, Meyer, III, Harry M, and Kilbey, II, S Michael. Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-containing Surface Scaffolds. United States: N. p., 2009. Web. doi:10.1021/la802925g.
Barrninger, Joshua, Messman, Jamie M, Banaszak, Abigail, Meyer, III, Harry M, & Kilbey, II, S Michael. Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-containing Surface Scaffolds. United States. https://doi.org/10.1021/la802925g
Barrninger, Joshua, Messman, Jamie M, Banaszak, Abigail, Meyer, III, Harry M, and Kilbey, II, S Michael. 2009. "Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-containing Surface Scaffolds". United States. https://doi.org/10.1021/la802925g.
@article{osti_948853,
title = {Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-containing Surface Scaffolds},
author = {Barrninger, Joshua and Messman, Jamie M and Banaszak, Abigail and Meyer, III, Harry M and Kilbey, II, S Michael},
abstractNote = {We describe the successful development of a procedure for the step-by-step formation of a reactive, multi-layer polymer scaffold incorporating polymers based on 2-vinyl-4,4-dimethylazlactone (VDMA) on a silicon wafer and the characterization of these materials. Also discussed is the development of a procedure for the non-site specific attachment of a biomolecule to the modified silicon wafer, including scaffolds modified via drop-on-demand (DOD) inkjet printing. VDMA-based polymers were used because of their hydrolytic stability and ability of the pendant azlactone rings to form stable covalent bonds with primary amines without byproducts via nucleophilic addition. This reaction proceeds without a catalyst and at room temperature, yielding a stable amide linkage, which adds to the ease of construction expected when using VDMA-based polymers. DOD inkjet printing was explored as an interesting method for creating surfaces with one or more patterns of biomolecules, because of the flexibility and ease in pattern design.},
doi = {10.1021/la802925g},
url = {https://www.osti.gov/biblio/948853}, journal = {Langmuir},
issn = {0743-7463},
number = 1,
volume = 25,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2009},
month = {Thu Jan 01 00:00:00 EST 2009}
}