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Title: Evaluation of Sampling Tools for Environmental Sampling of Bacterial Endospores from Porous and Non-porous Surfaces

Aims: Having and executing a well-defined and validated sampling protocol is critical following a purposeful release of a biological agent for response and recovery activities, for clinical and epidemiological analysis and for forensic purposes. The objective of this study was to address the need for validated sampling and analysis methods called out by the General Accounting Office and others to systematically compare the collection efficiency of various swabs and wipes for collection of bacterial endospores from five different surfaces, both porous and non-porous. This study was also designed to test the collection and extraction solutions used for endospore recovery from swabs and wipes. Methods and Results: Eight collection tools were used, five swabs and three wipes. Three collection/preservation solutions were evaluated: sterile E-pureĀ® water, phosphate buffered saline (PBS), and phosphate buffered saline with 0.3% Tween (PBST). An Ink Jet Aerosol Generator (IJAG) was used to apply Bacillus subtilis endospores to five porous and non-porous surfaces. The collection efficiencies of the swabs and wipes were compared using a statistical multiple comparison analysis. Results indicated that wipes tend to have higher collection efficiency than swabs. Of the swabs tested, the recovery from most of the surfaces was highest with the polyurethane foammore » swab. Conclusions: The ScottPureĀ® wipe had the highest collection efficiency and PBST was the best collection solution of those tested. Significance and Impact of Study: Validated sampling for potential biological warfare is of significant importance and this study answered some relevant questions.« less
Authors:
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Publication Date:
OSTI Identifier:
948405
Report Number(s):
PNNL-SA-60777
Journal ID: ISSN 1364-5072; JAMIFK; TRN: US200906%%329
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Applied Microbiology, 105 (4):1107-1113; Journal Volume: 105; Journal Issue: 4
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; AEROSOL GENERATORS; BACILLUS SUBTILIS; BIOLOGICAL WARFARE; EFFICIENCY; EVALUATION; PHOSPHATES; POLYURETHANES; SAMPLING; US GAO; WATER