Determination of chemical groups responsible for complexation of Cd and U to bacterial surface with pH.

Conference · Mon Jan 01 00:00:00 EST 2001 · J. Synchrotron Rad.

DOI:https://doi.org/10.1107/S0909049500021014· OSTI ID:943010

Kelly, S D; Boyanov, M; Bunker, B A; Fein, J B; Fowle, D A; Kemner, K M

Bacteria, which are ubiquitous in near-surface geologic systems, can affect the distribution and fate of metals in these systems through adsorption reactions between the metals and bacterial cell walls. Recently, Fein et al. (1997) developed a chemical equilibrium approach to quantify metal adsorption onto cell walls, treating the sorption as a surface complexation phenomenon. However, such models are based on circumstantial bulk adsorption evidence only, and the nature and mechanism of metal binding to cell walls for each metal system have not been determined spectroscopically. The results of XAFS measurements at the Cd K-edge and U L3-edge on Bacillus-subtilis exposed to these elements show that, at low pH, U binds to phosphoryl groups while Cd binds to carboxyl functional groups.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States)
• Sponsoring Organization: USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program
• DOE Contract Number: DE-AC02-06CH11357
• OSTI ID: 943010
• Report Number(s): ANL/ER/CP-36668; JSYRES; TRN: US201002%%504
• Journal Information: J. Synchrotron Rad., Vol. 8, Issue 2001; Conference: 11th International XAFS Conference; Jul. 26, 2000 - Jul. 31, 2000; Ako, Japan; ISSN 0909-0495
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ABSORPTION SPECTROSCOPY
ADSORPTION
BACILLUS SUBTILIS
BACTERIA
CELL WALL
CADMIUM
DISTRIBUTION
ELEMENTS
EQUILIBRIUM
FINE STRUCTURE
FUNCTIONALS
FUNCTIONS
METALS
SORPTION
SURFACES
URANIUM
X-RAY SPECTROSCOPY