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Title: Structural determination of intact proteins using mass spectrometry

The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments in the mass spectrometer, eliminating complex microseparations, and protein preparatory chemical steps necessary for cross-linking based protein structure determination.Additionally, the preferred method of the present invention involves the determination of protein structures utilizing a top-down analysis of protein structures to search for covalent modifications. In the preferred method, intact proteins are ionized and fragmented within the mass spectrometer.
Authors:
 [1] ;  [2] ;  [3]
  1. (San Francisco, CA)
  2. (Oakland, CA)
  3. (Livermore, CA)
Publication Date:
OSTI Identifier:
936326
Report Number(s):
7,368,290
US patent application 10/437,268
DOE Contract Number:
AC04-94AL85000
Resource Type:
Patent
Research Org:
Sandia National Laboratories (SNL-CA), Livermore, CA
Sponsoring Org:
United States Department of Energy
Country of Publication:
United States
Language:
English