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Title: Chemical imaging of biological materials by NanoSIMS using isotopic and elemental labels

The NanoSIMS 50 combines unprecedented spatial resolution (as good as 50 nm) with ultra-high sensitivity (minimum detection limit of {approx}200 atoms). The NanoSIMS 50 incorporates an array of detectors, enabling simultaneous collection of 5 species originating from the same sputtered volume of a sample. The primary ion beam (Cs{sup +} or O{sup -}) can be scanned across the sample to produce quantitative secondary ion images. This capability for multiple isotope imaging with high spatial resolution provides a novel new approach to the study of biological materials. Studies can be made of sub-regions of tissues, mammalian cells, and bacteria. Major, minor and trace element distributions can be mapped on a submicron scale, growth and metabolism can be tracked using stable isotope labels, and biogenic origin can be determined based on composition. We have applied this technique extensively to mammalian and prokaryotic cells and bacterial spores. The NanoSIMS technology enables the researcher to interrogate the fate of molecules of interest within cells and organs through elemental and isotopic labeling. Biological applications at LLNL will be discussed.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
928540
Report Number(s):
UCRL-PROC-220500
TRN: US200811%%390
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Presented at: 19th Annual Workshop on SIMS, Rancho Mirage, CA, United States, May 16 - May 19, 2006
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; ATOMS; BACTERIA; BACTERIAL SPORES; BIOLOGICAL MATERIALS; ELEMENTS; ION BEAMS; LAWRENCE LIVERMORE NATIONAL LABORATORY; METABOLISM; ORGANS; ORIGIN; SENSITIVITY; SPATIAL RESOLUTION; STABLE ISOTOPES; TRACE AMOUNTS