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Title: Synthesis of Lutetium Phosphate/Apoferritin Core-Shell Nanoparticles for Potential Applications in Radioimmunoimaging and Radioimmunotherapy of Cancers

Abstract

We report a novel approach for synthesizing LuPO4/apoferritin core-shell nanoparticles based on an apoferritin template, conjugated to the protein biotin. To prepare the nanoparticle conjugates, we used non-radioactive lutetium as a model target or surrogate for radiolutetium (177Lu). The central cavity, multi-channel structure, and chemical properties of apoferritin are well-suited for sequentially diffusing lutetium and phosphate ions into the cavity--resulting in a stable core-shell composite. We characterized the synthesized LuPO4/apoferritin nanoparticle using transmission electron microscopy (TEM) and x-ray photoelectron spectroscopy (XPS). We tested the pre-targeting capability of biotin-modified lutetium/apoferritin nanoparticle using streptavidin-modified magnetic beads and streptavidin-modified fluorescein isothiocyanate (FITC) tracer. This paper presents a simple, fast, and efficient method for synthesizing LuPO4/apoferritin nanoparticle conjugates with biotin for potential applications in radioimmunotherapy and radioimmunoimaging of cancer.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
927974
Report Number(s):
PNNL-SA-59270
Journal ID: ISSN 0959-9428; JMACEP; 24822; TRN: US0804697
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of Materials Chemistry, 18(15):1779-1783
Additional Journal Information:
Journal Volume: 18; Journal Issue: 15; Journal ID: ISSN 0959-9428
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; BIOTIN; CHEMICAL PROPERTIES; FLUORESCEIN; ISOTHIOCYANATES; LUTETIUM; NEOPLASMS; PHOSPHATES; PROTEINS; RADIOIMMUNOTHERAPY; SYNTHESIS; TARGETS; TRANSMISSION ELECTRON MICROSCOPY; X-RAY PHOTOELECTRON SPECTROSCOPY; Apoferritin; lutetium-177; nanoparticles; radioimmunotherapy; radioimmunoimaging; Environmental Molecular Sciences Laboratory

Citation Formats

Wu, Hong, Engelhard, Mark H, Wang, Jun, Fisher, Darrell R, and Lin, Yuehe. Synthesis of Lutetium Phosphate/Apoferritin Core-Shell Nanoparticles for Potential Applications in Radioimmunoimaging and Radioimmunotherapy of Cancers. United States: N. p., 2008. Web. doi:10.1039/b717613a.
Wu, Hong, Engelhard, Mark H, Wang, Jun, Fisher, Darrell R, & Lin, Yuehe. Synthesis of Lutetium Phosphate/Apoferritin Core-Shell Nanoparticles for Potential Applications in Radioimmunoimaging and Radioimmunotherapy of Cancers. United States. https://doi.org/10.1039/b717613a
Wu, Hong, Engelhard, Mark H, Wang, Jun, Fisher, Darrell R, and Lin, Yuehe. 2008. "Synthesis of Lutetium Phosphate/Apoferritin Core-Shell Nanoparticles for Potential Applications in Radioimmunoimaging and Radioimmunotherapy of Cancers". United States. https://doi.org/10.1039/b717613a.
@article{osti_927974,
title = {Synthesis of Lutetium Phosphate/Apoferritin Core-Shell Nanoparticles for Potential Applications in Radioimmunoimaging and Radioimmunotherapy of Cancers},
author = {Wu, Hong and Engelhard, Mark H and Wang, Jun and Fisher, Darrell R and Lin, Yuehe},
abstractNote = {We report a novel approach for synthesizing LuPO4/apoferritin core-shell nanoparticles based on an apoferritin template, conjugated to the protein biotin. To prepare the nanoparticle conjugates, we used non-radioactive lutetium as a model target or surrogate for radiolutetium (177Lu). The central cavity, multi-channel structure, and chemical properties of apoferritin are well-suited for sequentially diffusing lutetium and phosphate ions into the cavity--resulting in a stable core-shell composite. We characterized the synthesized LuPO4/apoferritin nanoparticle using transmission electron microscopy (TEM) and x-ray photoelectron spectroscopy (XPS). We tested the pre-targeting capability of biotin-modified lutetium/apoferritin nanoparticle using streptavidin-modified magnetic beads and streptavidin-modified fluorescein isothiocyanate (FITC) tracer. This paper presents a simple, fast, and efficient method for synthesizing LuPO4/apoferritin nanoparticle conjugates with biotin for potential applications in radioimmunotherapy and radioimmunoimaging of cancer.},
doi = {10.1039/b717613a},
url = {https://www.osti.gov/biblio/927974}, journal = {Journal of Materials Chemistry, 18(15):1779-1783},
issn = {0959-9428},
number = 15,
volume = 18,
place = {United States},
year = {Tue Apr 01 00:00:00 EDT 2008},
month = {Tue Apr 01 00:00:00 EDT 2008}
}