Resolution of coi-dominant phytoplankton species in a eutrophiclake using synchrotron-based Fourier transform infraredspectroscopy
Synchrotron-based Fourier-transform infrared (FTIR)microspectroscopy was used to distinguish micropopulations of thecodominant algae Microcystis aeruginosa (Cyanophyceae) and Ceratiumhirundinella (Dinophyceae) in mixed phytoplankton samples taken from thewater column of a stratified eutrophic lake (Rostherne Mere, UK). FTIRspectra of the two algae showed a closely similar sequence of 10 bandsover the wave-number range 4000-900 cm-1. These were assigned to a rangeof vibrationally active chemical groups using published band assignmentsand on the basis of correlation and factor analysis. In both algae,intracellular concentrations of macromolecular components (determined asband intensity) varied considerably within the same population,indicating substantial intraspecific heterogeneity. Interspecificdifferences were separately analysed in relation to discrete bands and bymultivariate analysis of the entire spectral region 1750-900 cm-1. Interms of discrete bands, comparison of individual intensities (normalisedto amide 1) demonstrated significant (99 percent probability level)differences in relation to six bands between the two algal species. Keyinterspecific differences were also noted in relation to the positions ofbands 2, 10 (carbohydrate) and 7 (protein) and in the 3-D plots derivedby principal component analysis (PCA) of the sequence of bandintensities. PCA of entire spectral regions showed clear resolutionofspecies in the PCA plot, with indication of separation on the basis ofprotein (region 1700-1500 cm1) and carbohydrate (region 1150-900 cm1)composition in the loading plot. Hierarchical cluster analysis (Wardalgorithm) of entire spectral regions also showed clear discrimination ofthe two species within the resulting dendrogram.
- Research Organization:
- COLLABORATION - U.Manchester/UK
- Sponsoring Organization:
- USDOE Director. Office of Science. Basic Energy Sciences; ENVIROSYNCH. Central Laboratory of Research Councils K
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 926294
- Report Number(s):
- LBNL-62686; R&D Project: 458121; BnR: KC0204016; TRN: US200807%%630
- Journal Information:
- Phycologia, Vol. 46, Issue 2; Related Information: Journal Publication Date: 1 March 2007
- Country of Publication:
- United States
- Language:
- English
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