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Title: Autofluorescence dynamics during reperfusion following long-term renal ischemia in a rat model

Optical properties of near-surface kidney tissue were monitored in order to assess response during reperfusion to long (20 minutes) versus prolonged (150 minutes) ischemia in an in vivo rat model. Specifically, autofluorescence images of the exposed surfaces of both the normal and the ischemic kidneys were acquired during both injury and reperfusion alternately under 355 nm and 266 nm excitations. The temporal profile of the emission of the injured kidney during the reperfusion phase under 355 nm excitation was normalized to that under 266 nm as a means to account for changes in tissue optical properties independent of ischemia as well as changes in the illumination/collection geometrical parameters in future clinical implementation of this technique using a hand-held probe. The scattered excitation light signal was also evaluated as a reference signal and found to be inadequate. Characteristic time constants were extracted using fit to a relaxation model and found to have larger mean values following 150 minutes of injury. The mean values were then compared with the outcome of a chronic survival study where the control kidney had been removed. Rat kidneys exhibiting longer time constants were much more likely to fail. This may lead to a method to assessmore » kidney viability and predict its ability to recover in the initial period following transplantation or resuscitation.« less
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
926019
Report Number(s):
LLNL-PROC-401369
TRN: US200807%%468
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Presented at: Photonics West, San Jose, CA, United States, Jan 18 - Jan 25, 2008
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EXCITATION; IMPLEMENTATION; IN VIVO; ISCHEMIA; KIDNEYS; OPTICAL PROPERTIES; RELAXATION; VIABILITY