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Title: Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough.

Desulfovibrio vulgaris Hildenborough belongs to a class ofsulfate-reducing bacteria (SRB) and is found ubiquitously in nature.Given the importance of SRB-mediated reduction for bioremediation ofmetal ion contaminants, ongoing research on D. vulgaris has been in thedirection of elucidating regulatory mechanisms for this organism under avariety of stress conditions. This work presents a global view of thisorganism's response to elevated growth temperature using whole-celltranscriptomics and proteomics tools. Transcriptional response (1.7-foldchange or greater; Z>1.5) ranged from 1,135 genes at 15 min to 1,463genes at 120 min for a temperature up-shift of 13oC from a growthtemperature of 37oC for this organism and suggested both direct andindirect modes of heat sensing. Clusters of orthologous group categoriesthat were significantly affected included posttranslationalmodifications; protein turnover and chaperones (up-regulated); energyproduction and conversion (down-regulated), nucleotide transport,metabolism (down-regulated), and translation; ribosomal structure; andbiogenesis (down-regulated). Analysis of the genome sequence revealed thepresence of features of both negative and positive regulation whichincluded the CIRCE element and promoter sequences corresponding to thealternate sigma factors ?32 and ?54. While mechanisms of heat shockcontrol for some genes appeared to coincide with those established forEscherichia coli and Bacillus subtilis, the presence of unique controlschemes for several other genes was also evident. Analysis of proteinexpression levels usingmore » differential in-gel electrophoresis suggestedgood agreement with transcriptional profiles of several heat shockproteins, including DnaK (DVU0811), HtpG (DVU2643), HtrA (DVU1468), andAhpC (DVU2247). The proteomics study also suggested the possibility ofposttranslational modifications in the chaperones DnaK, AhpC, GroES(DVU1977), and GroEL (DVU1976) and also several periplasmic ABCtransporters.« less
Authors:
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Publication Date:
OSTI Identifier:
923002
Report Number(s):
LBNL--60298
Journal ID: ISSN 0021-9193; JOBAAY; TRN: US200804%%833
DOE Contract Number:
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Bacteriology; Journal Volume: 188; Journal Issue: 5; Related Information: Journal Publication Date: 2006
Research Org:
COLLABORATION - Collaboration with BiosystemsResearch Department Sandia National Laboratory; Environmental SciencesDivision, Oak Ridge National Laboratory; Physical Biosciences Division,Lawrence Be
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACILLUS SUBTILIS; BIOREMEDIATION; DESULFOVIBRIO; ELECTROPHORESIS; ESCHERICHIA COLI; GENES; GLOBAL ANALYSIS; HEAT-SHOCK PROTEINS; METABOLISM; MODIFICATIONS; NUCLEOTIDES; PROMOTERS; PROTEINS; REGULATIONS; SULFATE-REDUCING BACTERIA