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Title: Cleavage of nucleic acids

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. (Madison, WI)
Publication Date:
OSTI Identifier:
921022
Report Number(s):
7,306,917
US patent application 11/222,565
DOE Contract Number:
FG02-94ER81891
Resource Type:
Patent
Research Org:
Third Wave Technologies, Inc. (Madison, WI)
Sponsoring Org:
United States Department of Energy
Country of Publication:
United States
Language:
English