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Title: Integrated crystal mounting and alignment system for high-throughput biological crystallography

A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [8] ;  [9] ;  [10]
  1. (San Leandro, CA)
  2. (Richmond, CA)
  3. (Antioch, CA)
  4. (Moraga, CA)
  5. (Oakland, CA)
  6. (Berkeley, CA)
  7. (Lafayette, CA)
  8. (Walnut Creek, CA)
  9. (Chicago, IL)
  10. (La Jolla, CA)
Publication Date:
OSTI Identifier:
915744
Report Number(s):
7,274,769
US patent application 11/064,357
DOE Contract Number:
AC03-76SF00098
Resource Type:
Patent
Research Org:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
Sponsoring Org:
United States Department of Energy
Country of Publication:
United States
Language:
English