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Title: Online Aerosol Mass Spectrometry of Single Micrometer-Sized Particles Containing Poly(ethylene glycol)

Analysis of poly(ethylene glycol)(PEG)-containing particles by online single particle aerosol mass spectrometers equipped with laser desorption ionization (LDI) is reported. We demonstrate that PEG-containing particles are useful in the development of aerosol mass spectrometers because of their ease of preparation, low cost, and inherently recognizable mass spectra. Solutions containing millimolar quantities of PEGs were nebulized and, after drying, the resultant micrometer-sized PEG containing particles were sampled. LDI (266 nm) of particles containing NaCl and PEG molecules of average molecular weight <500 generated mass spectra reminiscent of mass spectra of PEG collected by other MS schemes including the characteristic distribution of positive ions (Na{sup +} adducts) separated by the 44 Da of the ethylene oxide units separating each degree of polymerization. PEGs of average molecular weight >500 were detected from particles that also contained t the tripeptide tyrosine-tyrosine-tyrosine or 2,5-dihydroxybenzoic acid, which were added to nebulized solutions to act as matrices to assist LDI using pulsed 266 nm and 355 nm lasers, respectively. Experiments were performed on two aerosol mass spectrometers, one reflectron and one linear, that each utilize two time-of-flight mass analyzers to detect positive and negative ions created from a single particle. PEG-containing particles are currently being employed inmore » the optimization of our bioaerosol mass spectrometers for the application of measurements of complex biological samples, including human effluents, and we recommend that the same strategies will be of great utility to the development of any online aerosol LDI mass spectrometer platform.« less
Authors:
; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
902327
Report Number(s):
UCRL-JRNL-227120
Journal ID: ISSN 0951-4198; RCMSEF; TRN: US200717%%255
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: Rapid Communications in Mass Spectrometry, vol. 21, no. 7, April 15, 2007, pp. 1214-1220; Journal Volume: 21; Journal Issue: 7
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ADDUCTS; AEROSOLS; ANIONS; CATIONS; DESORPTION; DISTRIBUTION; DRYING; ETHYLENE; IONIZATION; LASERS; MASS SPECTRA; MASS SPECTROMETERS; MASS SPECTROSCOPY; MATRICES; MOLECULAR WEIGHT; OPTIMIZATION; OXIDES; POLYMERIZATION