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Title: Mutagenic and antimutagenic effects of air-borne particulate extracts in salmonella typhimurium strains

Abstract

The presence of mutagens among crude airborne particulate extracts, by the Ames bioassay, has been recorded in a variety of geographic loci. However, the role such complex mixtures play in producing synergistic or antagonistic effects on mutagenesis has not been adequately investigated. To establish mutagenic interaction patterns of these agents samples of acetone extracts of crude and size-classified particulates were tested for mutagenicity by the Ames bioassay with tester strains TA100 and TA98. A dose-response mutagenicity was visible in the crude and size-classified particulate extracts. Both direct and indirect acting frameshift and base-substitution mutagens were detected. Analysis of the data with respect to synergism and antagonism indicate that the mutagenic activity of benzo(a)pyrene (BAP) (2ug/plate) and 2-aminoanthracene (2AA) (1.5 ug/plate) is antagonized significantly by mutagenic and non-mutagenic crude particulate extracts (400ug/plate). The same concentration of crude extracts markedly increased cell survival to BAP and 2AA. Size-fractionation demonstrated that the antimutagenic potency over the crude extract increased appreciably as the particle size decreased, with particles of less than 1.5 um showing the greatest antigenotoxic effect.

Authors:
;  [1]
  1. Duquesne Univ., Pittsburg, PA (United States)
Publication Date:
OSTI Identifier:
88915
Report Number(s):
CONF-9405324-
Journal ID: EMMUEG; ISSN 0893-6692; CNN: Grant 79-4; TRN: 95:004220-0060
Resource Type:
Journal Article
Journal Name:
Environmental and Molecular Mutagenesis
Additional Journal Information:
Journal Volume: 23; Journal Issue: Suppl.23; Conference: 25. annual meeting of the Environmental Mutagen Society, Portland, OR (United States), 7-12 May 1994; Other Information: PBD: 1994
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; PARTICULATES; MUTAGEN SCREENING; DOSE-RESPONSE RELATIONSHIPS; BENZOPYRENE; BIOLOGICAL EFFECTS; ANTHRACENE; PARTICLE SIZE; SYNERGISM

Citation Formats

Sideropoulus, A S, and Specht, S M. Mutagenic and antimutagenic effects of air-borne particulate extracts in salmonella typhimurium strains. United States: N. p., 1994. Web.
Sideropoulus, A S, & Specht, S M. Mutagenic and antimutagenic effects of air-borne particulate extracts in salmonella typhimurium strains. United States.
Sideropoulus, A S, and Specht, S M. 1994. "Mutagenic and antimutagenic effects of air-borne particulate extracts in salmonella typhimurium strains". United States.
@article{osti_88915,
title = {Mutagenic and antimutagenic effects of air-borne particulate extracts in salmonella typhimurium strains},
author = {Sideropoulus, A S and Specht, S M},
abstractNote = {The presence of mutagens among crude airborne particulate extracts, by the Ames bioassay, has been recorded in a variety of geographic loci. However, the role such complex mixtures play in producing synergistic or antagonistic effects on mutagenesis has not been adequately investigated. To establish mutagenic interaction patterns of these agents samples of acetone extracts of crude and size-classified particulates were tested for mutagenicity by the Ames bioassay with tester strains TA100 and TA98. A dose-response mutagenicity was visible in the crude and size-classified particulate extracts. Both direct and indirect acting frameshift and base-substitution mutagens were detected. Analysis of the data with respect to synergism and antagonism indicate that the mutagenic activity of benzo(a)pyrene (BAP) (2ug/plate) and 2-aminoanthracene (2AA) (1.5 ug/plate) is antagonized significantly by mutagenic and non-mutagenic crude particulate extracts (400ug/plate). The same concentration of crude extracts markedly increased cell survival to BAP and 2AA. Size-fractionation demonstrated that the antimutagenic potency over the crude extract increased appreciably as the particle size decreased, with particles of less than 1.5 um showing the greatest antigenotoxic effect.},
doi = {},
url = {https://www.osti.gov/biblio/88915}, journal = {Environmental and Molecular Mutagenesis},
number = Suppl.23,
volume = 23,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}