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Title: Fluorescence Anisotropy Studies of Molecularly Imprinted Polymers

A molecularly imprinted polymer (MIP) is a biomimetic material that can be used as a biochemical sensing element. We studied the steady-state and time-resolved fluorescence and fluorescence anisotropy of anthracene imprinted polyurethane. We compared MIPs with imprinted analytes present, MIPs with the imprinted analytes extracted, MIPs with rebound analytes, non-imprinted control polymers (non-MIPs), and non-MIPs bound with analytes to understand MIP’s binding behavior. MIPs and non-MIPs had similar steady-state fluorescence anisotropy in the range of 0.11–0.24. Anthracene rebound in MIPs and non-MIPs had a fluorescence lifetime _=0.64 ns and a rotational correlation time _F =1.2–1.5 ns, both of which were shorter than that of MIPs with imprinted analytes present (_=2.03 ns and _F =2.7 ns). The steady-state anisotropy of polymer solutions increased exponentially with polymerization time and might be used to characterize the polymerization extent in-situ.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
878268
Report Number(s):
PNNL-SA-48527
3532; KP1704020; TRN: US200611%%14
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Luminescence, 21(1):7-14; Journal Volume: 21; Journal Issue: 1
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ANISOTROPY; ANTHRACENE; FLUORESCENCE; POLYMERIZATION; POLYURETHANES Environmental Molecular Sciences Laboratory