skip to main content

Title: Kit for detecting nucleic acid sequences using competitive hybridization probes

A kit is provided for detecting a target nucleic acid sequence in a sample, the kit comprising: a first hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the first hybridization probe including a first complexing agent for forming a binding pair with a second complexing agent; and a second hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the first hybridization probe does not selectively hybridize, the second hybridization probe including a detectable marker; a third hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the third hybridization probe including the same detectable marker as the second hybridization probe; and a fourth hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the third hybridization probe does not selectively hybridize, the fourth hybridization probe including the first complexing agent for forming a binding pair with the second complexing agent; whereinmore » the first and second hybridization probes are capable of simultaneously hybridizing to the target sequence and the third and fourth hybridization probes are capable of simultaneously hybridizing to the target sequence, the detectable marker is not present on the first or fourth hybridization probes and the first, second, third, and fourth hybridization probes each include a competitive nucleic acid sequence which is sufficiently complementary to a third portion of the target sequence that the competitive sequences of the first, second, third, and fourth hybridization probes compete with each other to hybridize to the third portion of the target sequence.« less
Authors:
 [1] ;  [2] ;  [3]
  1. (San Ramon, CA)
  2. (Tracy, CA)
  3. (Walnut Creek, CA)
Publication Date:
OSTI Identifier:
873914
Report Number(s):
US 6270972
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Patent
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Country of Publication:
United States
Language:
English
Subject:
kit; detecting; nucleic; acid; sequences; competitive; hybridization; probes; kit; provided; detecting; target; nucleic; acid; sequence; sample; kit; comprising; hybridization; probe; nucleic; acid; sequence; sufficiently; complementary; selectively; hybridize; portion; target; sequence; hybridization; probe; including; complexing; agent; forming; binding; pair; complexing; agent; hybridization; probe; nucleic; acid; sequence; sufficiently; complementary; selectively; hybridize; portion; target; sequence; hybridization; probe; selectively; hybridize; hybridization; probe; including; detectable; marker; third; hybridization; probe; nucleic; acid; sequence; sufficiently; complementary; selectively; hybridize; portion; target; sequence; third; hybridization; probe; including; detectable; marker; hybridization; probe; fourth; hybridization; probe; nucleic; acid; sequence; sufficiently; complementary; selectively; hybridize; portion; target; sequence; third; hybridization; probe; selectively; hybridize; fourth; hybridization; probe; including; complexing; agent; forming; binding; pair; complexing; agent; hybridization; probes; capable; simultaneously; hybridizing; target; sequence; third; fourth; hybridization; probes; capable; simultaneously; hybridizing; target; sequence; detectable; marker; fourth; hybridization; probes; third; fourth; hybridization; probes; competitive; nucleic; acid; sequence; sufficiently; complementary; third; portion; target; sequence; competitive; sequences; third; fourth; hybridization; probes; compete; hybridize; third; portion; target; sequence; detecting nucleic; detectable marker; detectable marker; detectable marker; probe including; probe including; probe including; probe including; acid sequence; acid sequence; acid sequence; acid sequence; acid sequence; acid sequence; acid sequence; nucleic acid; nucleic acid; nucleic acid; nucleic acid; nucleic acid; nucleic acid; nucleic acid; acid sequences; target sequence; target sequence; target sequence; target sequence; target sequence; target sequence; target sequence; target sequence; target nucleic; complexing agent; complexing agent; complexing agent; complexing agent; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probe; hybridization probes; hybridization probes; hybridization probes; hybridization probes; hybridization probes; hybridization probes; sufficiently complementary; sufficiently complementary; sufficiently complementary; sufficiently complementary; sufficiently complementary; binding pair; binding pair; target nuclei; competitive hybridization /435/999/