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Title: Nucleic acid indexing

A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.
Authors:
 [1] ;  [2]
  1. (Madison, WI)
  2. (Bellevue, WA)
Publication Date:
OSTI Identifier:
872707
Report Number(s):
US 5994068
DOE Contract Number:
FG02-91ER61122
Resource Type:
Patent
Research Org:
University of Wisconsin
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; indexing; restriction; site; method; selectively; amplifying; fragment; generated; ii; enzyme; adaptors; specific; containing; adaptor; sequences; complementary; near; termini; fragments; cleavage; combinatorial; facilitates; amplification; sequence; nucleic acid; restriction enzyme; restriction fragments; restriction site; indexing sequences; selectively amplifying; sequences complementary; site indexing; fragments generated; fragment generated; fragment indexing; indexing method; ii restriction; restriction fragment; acid indexing; adaptors specific; adaptor indexing; containing adaptor; /435/999/