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Title: Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology

An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.
Authors:
 [1]
  1. (Knoxville, TN)
Publication Date:
OSTI Identifier:
872543
Report Number(s):
US 5958740
DOE Contract Number:
AC05-96OR22464
Resource Type:
Patent
Research Org:
LOCKHEED MARTIN ENERGY RES COR
Country of Publication:
United States
Language:
English
Subject:
genetically; enhanced; cellulase; production; pseudomonas; cellulosa; recombinant; dna; technology; enhanced; strain; pseudomonas; celllulosa; obtained; introducing; recombinant; genetic; construct; comprising; heterologous; cellulase; operably; connected; promoter; atcc; 55702; mutagenizing; transformants; treatment; mnng; selecting; cellulase; producing; transformant; transformant; designated; pseudomonas; cellulosa; atcc; xxxx; exhibits; enhanced; levels; cellulase; production; relative; untransformed; pseudomonas; cellulosa; strain; 142; atcc; 55702; cellulase producing; enhanced cellulase; recombinant dna; operably connected; exhibits enhanced; construct comprising /435/424/