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Title: Recombinant cells that highly express chromosomally-integrated heterologous genes

Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
Authors:
 [1] ;  [1] ;  [1]
  1. (Gainesville, FL)
Publication Date:
OSTI Identifier:
871892
Report Number(s):
US 5821093
DOE Contract Number:
FG05-86ER13574
Resource Type:
Patent
Research Org:
University of Florida (Gainesville, FL)
Country of Publication:
United States
Language:
English
Subject:
recombinant; cells; highly; express; chromosomally-integrated; heterologous; genes; recombinant; host; cells; obtained; comprise; heterologous; polypeptide-encoding; polynucleotide; segment; stably; integrated; chromosome; transcriptional; control; endogenous; promoter; mutation; effects; increased; expression; heterologous; segment; resulting; enhanced; production; host; cells; polypeptide; encoded; segment; relative; production; polypeptide; host; cells; absence; mutation; increased; expression; achieved; retained; absence; conditions; select; cells; displaying; increased; expression; integrated; segment; comprises; example; ethanol-production; genes; efficient; ethanol; producer; zymomonas; mobilis; recombinant; escherichia; coli; enteric; bacterial; cells; capable; converting; wide; range; biomass-derived; sugars; efficiently; ethanol; zymomonas mobilis; wide range; host cells; host cells; host cells; recombinant host; host cell; host cell; host cell; recombinant cells; segment comprises; escherichia coli; heterologous genes; stably integrated; chromosomally-integrated heterologous; express chromosomally-integrated; highly express; polypeptide-encoding polynucleotide; polynucleotide segment /435/999/