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Title: Surface enhanced Raman gene probe and methods thereof

The subject invention disclosed herein is a new gene probe biosensor and methods thereof based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays.
Authors:
 [1]
  1. (Knoxville, TN)
Publication Date:
OSTI Identifier:
871728
Report Number(s):
US 5783389
DOE Contract Number:
AC05-84OR21400
Resource Type:
Patent
Research Org:
LOCKHEED MARTIN ENRGY SYST INC
Country of Publication:
United States
Language:
English
Subject:
surface; enhanced; raman; probe; methods; subject; disclosed; probe; biosensor; methods; based; surface; enhanced; raman; scattering; sers; label; detection; ser; probe; biosensor; comprises; support; means; ser; probe; oligonucleotide; strand; labeled; sers; label; sers; active; substrate; disposed; support; means; ser; probes; adsorbed; thereon; biotargets; bacterial; viral; dna; rna; pna; detected; ser; probe; via; hybridization; oligonucleotide; strands; complementary; ser; probe; support; means; supporting; sers; active; substrate; fiberoptic; probe; array; fiberoptic; probes; performance; multiple; assays; waveguide; microsensor; array; charge-coupled; devices; photodiode; arrays; sers active; sers active; probe biosensor; probe biosensor; oligonucleotide strand; oligonucleotide strand; sers label; active substrate; active substrate; diode arrays; photodiode array; charge-coupled device; means supporting; photodiode arrays; substrate disposed; sensor array; coupled device; raman scattering; support means; support means; support means; diode array; sensor comprises; enhanced raman; enhanced raman; optic probe; optic probe; surface enhanced; surface enhanced; fiberoptic probe; fiberoptic probe; waveguide microsensor; strand labeled; strands complementary; viral dna; via hybridization; probes adsorbed; microsensor array; multiple assays; oligonucleotide strands; biosensor comprises; charge-coupled devices; adsorbed thereon; probe via; nucleotide strand; nucleotide strand; nucleotide strands; fiberoptic probes; label detection; optic probes /435/