skip to main content

Title: Normalized cDNA libraries

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
Authors:
 [1] ;  [2]
  1. (New York, NY)
  2. (Englewood, NJ)
Publication Date:
OSTI Identifier:
870993
Report Number(s):
US 5637685
US patent application 08/465811
DOE Contract Number:
FG02-91ER61233
Resource Type:
Patent
Research Org:
Columbia University
Country of Publication:
United States
Language:
English
Subject:
normalized; cdna; libraries; provides; method; normalize; directional; cdna; library; constructed; vector; allows; propagation; single-stranded; circle; form; comprising; propagating; directional; cdna; library; single-stranded; circles; generating; fragments; complementary; noncoding; sequence; single-stranded; circles; library; produce; partial; duplexes; purifying; partial; duplexes; melting; reassociating; purified; partial; duplexes; moderate; cot; purifying; unassociated; single-stranded; circles; generating; normalized; cdna; library; cdna libraries; produce partial; directional cdna; directional cdna; single-stranded circles; single-stranded circles; single-stranded circles; cdna library; cdna library; cdna library; partial duplexes; partial duplexes; partial duplexes; normalized cdna; normalized cdna; purified partial; single-stranded circle; single-stranded circle; single-stranded circle; single-stranded circle; unassociated single-stranded; noncoding sequence; circle form; allows propagation; coding sequence; library constructed; form comprising; generating fragments; fragments complementary /999/435/