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Title: Normalized cDNA libraries

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
 [1] ;  [2]
  1. (New York, NY)
  2. (Englewood, NJ)
Publication Date:
OSTI Identifier:
Report Number(s):
US 5637685
US patent application 08/465811
DOE Contract Number:
Resource Type:
Research Org:
Columbia University
Country of Publication:
United States
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