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Title: Ethanol production by recombinant hosts

Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [1]
  1. (Gainesville, FL)
  2. (Vicosa, BR)
  3. (Miyazaki, JP)
Publication Date:
OSTI Identifier:
869925
Report Number(s):
US 5424202
DOE Contract Number:
FG05-86ER13574
Resource Type:
Patent
Research Org:
University of Florida (Gainesville, FL)
Country of Publication:
United States
Language:
English
Subject:
ethanol; production; recombinant; hosts; novel; plasmids; comprising; genes; code; alcohol; dehydrogenase; pyruvate; decarboxylase; described; described; recombinant; hosts; transformed; genes; coding; alcohol; dehydrogenase; pyruvate; virtue; transformation; genes; recombinant; hosts; capable; producing; significant; amounts; ethanol; fermentation; product; disclosed; methods; increasing; growth; recombinant; hosts; methods; reducing; accumulation; undesirable; metabolic; products; growth; medium; hosts; disclosed; recombinant; host; capable; producing; significant; amounts; ethanol; fermentation; product; oligosaccharides; plasmids; comprising; genes; encoding; polysaccharases; addition; genes; described; code; alcohol; dehydrogenase; pyruvate; decarboxylase; methods; described; producing; ethanol; oligomeric; feedstock; recombinant; hosts; described; provided; method; enhancing; production; functional; proteins; recombinant; host; comprising; overexpressing; adhb; host; provided; process; designs; fermenting; oligosaccharide-containing; biomass; ethanol; fermentation product; fermentation product; comprising genes; comprising genes; plasmids comprising; plasmids comprising; ethanol production; producing ethanol; significant amounts; significant amounts; alcohol dehydrogenase; alcohol dehydrogenase; alcohol dehydrogenase; recombinant host; recombinant host; recombinant host; recombinant host; recombinant host; recombinant host; recombinant host; recombinant hosts; recombinant hosts; recombinant hosts; recombinant hosts; recombinant hosts; pyruvate decarboxylase; pyruvate decarboxylase; undesirable metabolic; metabolic products; significant amount; significant amount; growth medium; novel plasmids; functional proteins; genes coding; genes encoding; process design /435/